Aldehyde dehydrogenase 5 Antibody - BSA Free Summary
| Immunogen |
Recombinant protein encompassing a sequence within the center region of human Aldehyde dehydrogenase 5. The exact sequence is proprietary. |
| Localization |
Mitochondrion matrix |
| Predicted Species |
Rat (94%), Primate (99%). Backed by our 100% Guarantee. |
| Isotype |
IgG |
| Clonality |
Polyclonal |
| Host |
Rabbit |
| Gene |
ALDH1B1 |
| Purity |
Antigen Affinity-purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- Immunocytochemistry/ Immunofluorescence 1:100-1:1000
- Immunohistochemistry 1:100-1:1000
- Immunohistochemistry-Paraffin 1:100-1:1000
- Western Blot 1:500-1:3000
|
| Theoretical MW |
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer |
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol |
| Preservative |
0.01% Thimerosal |
| Purity |
Antigen Affinity-purified |
Alternate Names for Aldehyde dehydrogenase 5 Antibody - BSA Free
Background
This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. This gene does not contain introns in the coding sequence. The variation of this locus may affect the development of alcohol-related problems. [provided by RefSeq]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
Publications for Aldehyde dehydrogenase 5 Antibody (NBP1-32569) (0)
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Product General Protocols
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Video Protocols
FAQs for Aldehyde dehydrogenase 5 Antibody (NBP1-32569) (0)
Secondary Antibodies
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Isotype Controls
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Blogs on Aldehyde dehydrogenase 5
![Immunocytochemistry/Immunofluorescence: Aldehyde dehydrogenase 5 Antibody [NBP1-32569] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ALDH1B1 protein stained by ALDH1B1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Aldehyde-dehydrogenase-5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32569-img0005.jpg "Immunocytochemistry/Immunofluorescence: Aldehyde dehydrogenase 5 Antibody [NBP1-32569] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ALDH1B1 protein stained by ALDH1B1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: Aldehyde dehydrogenase 5 Antibody [NBP1-32569] - Paraffin-embedded mouse duodenum. ALDH1B1 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Aldehyde-dehydrogenase-5-Antibody-Immunohistochemistry-Paraffin-NBP1-32569-img0004.jpg "Immunohistochemistry-Paraffin: Aldehyde dehydrogenase 5 Antibody [NBP1-32569] - Paraffin-embedded mouse duodenum. ALDH1B1 antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: Aldehyde dehydrogenase 5 Antibody [NBP1-32569] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Aldehyde-dehydrogenase-5-Antibody-Immunohistochemistry-Paraffin-NBP1-32569-img0003.jpg "Immunohistochemistry-Paraffin: Aldehyde dehydrogenase 5 Antibody [NBP1-32569] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:500 dilution.")
![Western Blot: Aldehyde dehydrogenase 5 Antibody [NBP1-32569] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Aldehyde dehydrogenase 5 antibody (NBP1-32569) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32569_rabbit-polyclonal-aldehyde-dehydrogenase-5-antibody-910202420115096.jpg "Western Blot: Aldehyde dehydrogenase 5 Antibody [NBP1-32569] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Aldehyde dehydrogenase 5 antibody (NBP1-32569) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Aldehyde_Dehydrogenase_1-A1_AF5869_Immunocytochemistry_10308.jpg)
![Immunohistochemistry Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Aldehyde_Dehydrogenase_1-A1_AF5869_Immunohistochemistry_10638.jpg)
![Simple Western Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Aldehyde_Dehydrogenase_1A1_AF5869_Simple_Western_17067.jpg)
![Immunohistochemistry Neurophysin II/Arg-vasopressin Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af009_human-neurophysin-ii-affinity-purified-polyclonal-ab-immunohistochemistry-2812202013195.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")