ACSL4 Antibody (A-5): sc-271800

ACSL4 Antibody (A-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects ACSL4 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-ACSL4 antibody (A-5) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. ACSL4, a member of the acyl-CoA synthetase family, plays a crucial role in lipid metabolism by catalyzing the conversion of long-chain fatty acids into acyl-CoA derivatives, which are essential for the synthesis of cellular lipids and the β-oxidation pathway. This enzymatic activity drives energy production and maintains cellular lipid homeostasis, impacting processes such as membrane formation and signaling. ACSL4 is expressed in various tissues, including the brain, heart, and skeletal muscle, where regulation links to important physiological functions, including energy metabolism and inflammatory response modulation. ACSL4′s ability to preferentially activate specific fatty acids, such as arachidonic acid, highlights its significance in cellular signaling pathways and potential implications in metabolic disorders and diseases.

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ACSL4 Antibody (A-5) References:

1. Regulation by adrenocorticotropic hormone and arachidonate of the expression of acyl-CoA synthetase 4, an arachidonate-preferring enzyme expressed in steroidogenic tissues.  |  Cho, YY., et al. 2000. Biochem Biophys Res Commun. 274: 741-5. PMID: 10924347
2. Acyl-CoAs are functionally channeled in liver: potential role of acyl-CoA synthetase.  |  Muoio, DM., et al. 2000. Am J Physiol Endocrinol Metab. 279: E1366-73. PMID: 11093925
3. Abnormal uterus with polycysts, accumulation of uterine prostaglandins, and reduced fertility in mice heterozygous for acyl-CoA synthetase 4 deficiency.  |  Cho, YY., et al. 2001. Biochem Biophys Res Commun. 284: 993-7. PMID: 11409893
4. Exon/intron organization and transcription units of the human acyl-CoA synthetase 4 gene.  |  Minekura, H., et al. 2001. Biochem Biophys Res Commun. 286: 80-6. PMID: 11485311
5. Genomic organization and transcription units of the human acyl-CoA synthetase 3 gene.  |  Minekura, H., et al. 2001. Gene. 278: 185-92. PMID: 11707336
6. Do long-chain acyl-CoA synthetases regulate fatty acid entry into synthetic versus degradative pathways?  |  Coleman, RA., et al. 2002. J Nutr. 132: 2123-6. PMID: 12163649
7. ACSL4 dictates ferroptosis sensitivity by shaping cellular lipid composition.  |  Doll, S., et al. 2017. Nat Chem Biol. 13: 91-98. PMID: 27842070
8. ACSL4 deficiency confers protection against ferroptosis-mediated acute kidney injury.  |  Wang, Y., et al. 2022. Redox Biol. 51: 102262. PMID: 35180475
9. CD8+ T cells and fatty acids orchestrate tumor ferroptosis and immunity via ACSL4.  |  Liao, P., et al. 2022. Cancer Cell. 40: 365-378.e6. PMID: 35216678
10. ACSL4 promotes microglia-mediated neuroinflammation by regulating lipid metabolism and VGLL4 expression.  |  Zhou, X., et al. 2023. Brain Behav Immun. 109: 331-343. PMID: 36791893
11. Molecular characterization and expression of rat acyl-CoA synthetase 3.  |  Fujino, T., et al. 1996. J Biol Chem. 271: 16748-52. PMID: 8663269
12. Alternative translation initiation generates acyl-CoA synthetase 3 isoforms with heterogeneous amino termini.  |  Fujino, T., et al. 1997. J Biochem. 122: 212-6. PMID: 9276691

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ACSL4 Antibody (A-5) has 49 citations in a variety of scientific publications.

Primary antibodies like Anti-ACSL4 Antibody (A-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ACSL4 Antibody (A-5) Specifications

Alternate Product Name: ACSL4

Product Clone Name: A-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 623-675

Epitope Target: ACSL4

Epitope Species: human

Product Reactivity/Detection: short isoform and long isoform of ACSL4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 49 citations in various scientific publications

Available Conjugates/Forms for ACSL4 Antibody (A-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred ACSL4 antibody is ACSL4 Antibody (F-4): sc-365230.