BPTF/FALZ Antibody - BSA Free Summary
| Immunogen |
This antibody was developed against Recombinant Protein corresponding to amino acids: SKLSQLKSQQVAAAAHEANKLFKEGKEVLVVNSQGEISRLSTKKEVIMKGNINNYFKLGQEGKYRVYHNQYSTNSFALNKHQHREDHDKRRHLAHKFCLTPAGEFKWNGSVHGSKVLTISTLRLTITQL |
| Predicted Species |
Mouse (98%), Rat (98%). Backed by our 100% Guarantee. |
| Isotype |
IgG |
| Clonality |
Polyclonal |
| Host |
Rabbit |
| Gene |
BPTF |
| Purity |
Immunogen affinity purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
- Immunohistochemistry 1:50 - 1:200
- Immunohistochemistry-Paraffin 1:50 - 1:200
|
| Application Notes |
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
| Control Peptide |
|
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
PBS (pH 7.2) and 40% Glycerol |
| Preservative |
0.02% Sodium Azide |
| Purity |
Immunogen affinity purified |
Alternate Names for BPTF/FALZ Antibody - BSA Free
Background
FALZ/BPTF is a histone-binding component of NURF (nucleosome remodeling factor), a complex that catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. This gene was identified in brain homogenates from patients with Alzheimer's disease. Analysis of the original protein (fetal Alz-50 reactive clone 1, or FAC1), containing a DNA-binding domain, a zinc finger motif, and a C-terminal bromodomain, suggested it might play a role in the regulation of transcription during proliferation. High levels of FAC1 were detected in fetal brain and in patients with neurodegenerative diseases. This protein is highly similar to the largest subunit of the Drosophila NURF (nucleosome remodeling factor) complex.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for BPTF/FALZ Antibody (NBP1-84756) (0)
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for BPTF/FALZ Antibody (NBP1-84756). (Showing 1 - 1 of 1 FAQ).
-
I am interesting by anti-BPTF antibody. I would like to know if a large protein like that could be revealed in PVDF membrane? ..show answer..
- Regarding your question, nucleosome-remodeling factor subunit (BPTF) is a very large protein as you have also mentioned (338KD). There are some tips for efficient transfer of these proteins in western blot for instance: 1) Make sure to use a low percentage gel, for BPTF in particular I suggest you use 7% gel or even lower (5%). 2) You can use either Nitrocellulose or PVDF, to my knowledge the binding efficiency is higher for nitrocellulose while PVDF is a better choice of membrane if you are planning to do re-probing and stripping. If you are going to use PVDF, make sure to wet it in 100% methanol before use. Furthermore, PVDF membrane exhibits better binding efficiency of electroblotted material in the presence of SDS in the transfer buffer. However SDS added to facilitate transfer of large proteins should not exceed 0.05% as it will interfere with the binding of the protein to the membrane. 3) For transfer you can either use the semi-dry or wet transfer but you decide doing wet transfer, I strongly recommend you doing the transfer in low voltage (at 50v) for instance in order to obtain an efficient transfer. These are some suggestions I can provide for a better and more efficient transfer for large proteins like BPTF.
Secondary Antibodies
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Isotype Controls
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Additional BPTF/FALZ Products
Blogs on BPTF/FALZ
![Immunocytochemistry/Immunofluorescence: BPTF/FALZ Antibody [NBP1-84756] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/BPTF-FALZ-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84756-img0004.jpg "Immunocytochemistry/Immunofluorescence: BPTF/FALZ Antibody [NBP1-84756] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: BPTF/FALZ Antibody [NBP1-84756] - Staining of human skeletal muscle shows weak to moderate positivity in myocytes.](http://images.novusbio.com/fullsize/BPTF-FALZ-Antibody-Immunohistochemistry-Paraffin-NBP1-84756-img0010.jpg "Immunohistochemistry-Paraffin: BPTF/FALZ Antibody [NBP1-84756] - Staining of human skeletal muscle shows weak to moderate positivity in myocytes.")
![Immunohistochemistry-Paraffin: BPTF/FALZ Antibody [NBP1-84756] - Staining of human adrenal gland shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/BPTF-FALZ-Antibody-Immunohistochemistry-Paraffin-NBP1-84756-img0006.jpg "Immunohistochemistry-Paraffin: BPTF/FALZ Antibody [NBP1-84756] - Staining of human adrenal gland shows moderate cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: BPTF/FALZ Antibody [NBP1-84756] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/BPTF-FALZ-Antibody-Immunohistochemistry-Paraffin-NBP1-84756-img0007.jpg "Immunohistochemistry-Paraffin: BPTF/FALZ Antibody [NBP1-84756] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.")
![Immunohistochemistry-Paraffin: BPTF/FALZ Antibody [NBP1-84756] - Staining of human placenta shows weak to moderate nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/BPTF-FALZ-Antibody-Immunohistochemistry-Paraffin-NBP1-84756-img0008.jpg "Immunohistochemistry-Paraffin: BPTF/FALZ Antibody [NBP1-84756] - Staining of human placenta shows weak to moderate nuclear positivity in trophoblastic cells.")
![Immunohistochemistry-Paraffin: BPTF/FALZ Antibody [NBP1-84756] - Staining of human duodenum shows strong cytoplasmic positivity in a subset of glandular cells.](http://images.novusbio.com/fullsize/BPTF-FALZ-Antibody-Immunohistochemistry-Paraffin-NBP1-84756-img0009.jpg "Immunohistochemistry-Paraffin: BPTF/FALZ Antibody [NBP1-84756] - Staining of human duodenum shows strong cytoplasmic positivity in a subset of glandular cells.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")