Tom22 Antibody (J-31): sc-101286

Tom22 Antibody (J-31) is a mouse monoclonal IgG2a kappa light chain antibody that detects Tom22 protein in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Tom22 (J-31) antibody comes in a non-conjugated form, offering versatility for various experimental applications. Tom22 functions as a critical component of the mitochondrial preprotein translocase of the outer membrane (Tom) complex, essential for importing nucleus-encoded precursor proteins into mitochondria. This protein complex supports cellular energy production and metabolism by ensuring proper protein transport into mitochondria for processes like oxidative phosphorylation and apoptosis. The Tom machinery includes import receptors like Tom20 and Tom22, forming a heteromeric receptor complex that initiates protein insertion into the outer membrane and guides them to the general import pore (GIP). Tom22 serves as a crucial docking point for both Tom20 and GIP, enabling efficient preprotein translocation. Tom22′s N-terminal negatively charged region and putative transmembrane domain influence protein interactions and stability within the mitochondrial membrane. Human Tom22 gene resides on chromosome 22q12-q13, demonstrating evolutionary conservation across species.

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Tom22 Antibody (J-31) References:

1. Distribution of binding sequences for the mitochondrial import receptors Tom20, Tom22, and Tom70 in a presequence-carrying preprotein and a non-cleavable preprotein.  |  Brix, J., et al. 1999. J Biol Chem. 274: 16522-30. PMID: 10347216
2. Biogenesis of Tom40, core component of the TOM complex of mitochondria.  |  Rapaport, D. and Neupert, W. 1999. J Cell Biol. 146: 321-31. PMID: 10427088
3. Tom22 is a multifunctional organizer of the mitochondrial preprotein translocase.  |  van Wilpe, S., et al. 1999. Nature. 401: 485-9. PMID: 10519552
4. The TOM core complex: the general protein import pore of the outer membrane of mitochondria.  |  Ahting, U., et al. 1999. J Cell Biol. 147: 959-68. PMID: 10579717
5. The transport machinery for the import of preproteins across the outer mitochondrial membrane.  |  Ryan, MT., et al. 2000. Int J Biochem Cell Biol. 32: 13-21. PMID: 10661891
6. Identification of mammalian TOM22 as a subunit of the preprotein translocase of the mitochondrial outer membrane.  |  Saeki, K., et al. 2000. J Biol Chem. 275: 31996-2002. PMID: 10900208
7. Identification and functional analysis of human Tom22 for protein import into mitochondria.  |  Yano, M., et al. 2000. Mol Cell Biol. 20: 7205-13. PMID: 10982837
8. Recognition of mitochondrial targeting sequences by the import receptors Tom20 and Tom22.  |  Rimmer, KA., et al. 2011. J Mol Biol. 405: 804-18. PMID: 21087612
9. Analysis of individual mitochondria via fluorescent immunolabeling with Anti-TOM22 antibodies.  |  Taylor, TH., et al. 2014. Anal Bioanal Chem. 406: 1683-91. PMID: 24481619
10. Role of the intermembrane-space domain of the preprotein receptor Tom22 in protein import into mitochondria.  |  Court, DA., et al. 1996. Mol Cell Biol. 16: 4035-42. PMID: 8754801
11. Mitochondrial protein import. Tom40 plays a major role in targeting and translocation of preproteins by forming a specific binding site for the presequence.  |  Rapaport, D., et al. 1997. J Biol Chem. 272: 18725-31. PMID: 9228044
12. Functional analysis of human mitochondrial receptor Tom20 for protein import into mitochondria.  |  Yano, M., et al. 1998. J Biol Chem. 273: 26844-51. PMID: 9756929

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Tom22 Antibody (J-31) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-Tom22 Antibody (J-31) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Tom22 Antibody (J-31) Specifications

Alternate Product Name: Tom22

Product Clone Name: J-31

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-143

Epitope Target: Tom22

Epitope Species: human

Product Reactivity/Detection: Tom22

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for Tom22 Antibody (J-31): N/A

The preferred Tom22 antibody is Tom22 Antibody (1C9-2): sc-58308.