nSMase Antibody (2C9) - Azide and BSA Free Summary
| Description |
Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen |
SMPD2 (AAH00038.1, 1 a.a. ~ 423 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MKPNFSLRLRIFNLNCWGIPYLSKHRADRMRRLGDFLNQESFDLALLEEVWSEQDFQYLRQKLSPTYPAAHHFRSGIIGSGLCVFSKHPIQELTQHIYTLNGYPYMIHHGDWFSGKAVGLLVLHLSGMVLNAYVTHLHAEYNRQKDIYLAHRVAQAWELAQFIHHTSKKADVVLLCGDLNMHPEDLGCCLLKEWTGLHDAYLETRDFKGSEEGNTMVPKNCYVSQQELKPFPFGVRIDYVLYKAVSGFYISCKSFETTTGFDPHSGTPLSDHEALMATLFVRHSPPQQNPSSTHGPAERSPLMCVLKEAWTELGLGMAQARWWATFASYVIGLGLLLLALLCVLAAGGGAGEAAILLWTPSVGLVLWAGAFYLFHVQEVNGLYRAQAELQHVLGRAREAQDLGPEPQPALLLGQQEGDRTKEQ |
| Localization |
Cell Membrane |
| Specificity |
SMPD2 - sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase) |
| Isotype |
IgG3 Kappa |
| Clonality |
Monoclonal |
| Host |
Mouse |
| Gene |
SMPD2 |
| Purity |
IgG purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- ELISA
- Immunohistochemistry
- Immunohistochemistry-Paraffin
- Sandwich ELISA
- Western Blot 1:500
|
| Application Notes |
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA. |
| Publications |
|
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer |
In 1x PBS, pH 7.4 |
| Preservative |
No Preservative |
| Purity |
IgG purified |
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for nSMase Antibody (2C9) - Azide and BSA Free
Background
Sphingomyelin is also present in all eukaryotic cell membranes, especially the plasma membrane, and is particularly concentrated in the nervous system because sphingomyelin is a major component of myelin, the fatty insulation wrapped around nerve cells by Schwann cells or oligodendrocytes. Multiple Sclerosis is a disease characterised by deterioration of the myelin sheath, leading to impairment of nervous conduction. nSMase (Neutral sphingomyelinase; Sphingomyelin phosphodiesterase 2) converts sphingomyelin to ceramide. Although widely expressed in all tissues examined, except the spleen, high enzymatic activity occurs only in the brain. Mice lacking Smpd2 and Smpd3 are completely devoid of neutral SMase activity.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for nSMase Antibody (H00006610-M01)(1)
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Product General Protocols
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Video Protocols
FAQs for nSMase Antibody (H00006610-M01) (0)
Secondary Antibodies
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Isotype Controls
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![Immunohistochemistry-Paraffin: nSMase Antibody (2C9) [H00006610-M01] - Analysis of monoclonal antibody to SMPD2 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/nSMase-Antibody-2C9-Immunohistochemistry-Paraffin-H00006610-M01-img0004.jpg "Immunohistochemistry-Paraffin: nSMase Antibody (2C9) [H00006610-M01] - Analysis of monoclonal antibody to SMPD2 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.")
![Sandwich ELISA: nSMase Antibody (2C9) [H00006610-M01] - Detection limit for recombinant GST tagged SMPD2 is approximately 10ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/nSMase-Antibody-2C9-Sandwich-ELISA-H00006610-M01-img0005.jpg "Sandwich ELISA: nSMase Antibody (2C9) [H00006610-M01] - Detection limit for recombinant GST tagged SMPD2 is approximately 10ng/ml as a capture antibody.")
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