Novus Biologicals products are now on bio-techne.com

MED21 Antibody

Images

 
Image 1 of 3
(
Enlarge)
Western Blot: MED21 Antibody [NBP1-87006] - Analysis in control (vector only transfected HEK293T lysate) and MED21 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

  • Size / Catalog#QtyPrice
    NBP1-87006-25ul
    25ul
    $329.00
    4-6 Business Days
    NBP1-87006
    0.1 ml
    $499.00
    4-6 Business Days
Shipping $45.00 per order
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Datasheet
Reviews & Publications
Protocols & FAQs
Support & Research

MED21 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DAVNSLADQFCNAIGVLQQCGPPASFNNIQTAINKDQPANPTEEYAQLFAALIARTAKDIDVLIDSLPSEESTAALQAASLYKLEEENHEAATCLEDVVYRGDMLLEKIQSALADIAQSQ
Predicted Species
Mouse (99%), Rat (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MED21
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
MED21 Protein (NBP1-87006PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MED21 Antibody

  • hSrb7
  • mediator complex subunit 21SRB7 (suppressor of RNA polymerase B, yeast) homolog
  • RNA polymerase II complex component SRB7
  • RNA polymerase II holoenzyme component SRB7
  • RNAPII complex component SRB7
  • SRB7 suppressor of RNA polymerase B homolog
  • SRB7SRB7 suppressor of RNA polymerase B homolog (yeast)
  • SURB7mediator of RNA polymerase II transcription subunit 21

Background

MED21 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymera

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00009443-M01
Species: Hu
Applications: ELISA, KD, S-ELISA, WB
NBP1-81328
Species: Hu
Applications: ICC/IF, WB
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information


Product PDFs

  SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MED21 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol MED21