Glutathione Peroxidase 2/GPX2 Antibody

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Western Blot: Glutathione Peroxidase 2/GPX2 Antibody [NB100-1888] - Staining (0.5ug/ml) of Human Liver lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml
Datasheet
Reviews & Publications
Protocols & FAQs
Support & Research

Glutathione Peroxidase 2/GPX2 Antibody Summary

Immunogen
Peptide with sequence C-SLDGEKVDFN corresponding to internal region according to NP_002074.2.
Predicted Species
Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GPX2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 2 ug/ml
Application Notes
WB: Approx. 24 kDa band observed in human liver lysates (calculated MW of 21.9 kDa band according to NP_002074.2). Use in IHC-P reactivity reported in (PMID: 24562575).
Control
Human Liver Whole Tissue Lysate (Adult Whole Normal)
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Human Prostate Whole Tissue Lysate (Adult Whole Normal)
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Glutathione Peroxidase 2/GPX2 Overexpression Lysate
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Publications
Read Publications using
NB100-1888 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 19151108); Rat reactivity reported in scientific literature (PMID: 18056462).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Glutathione Peroxidase 2/GPX2 Antibody

  • EC 1.11.1
  • EC 1.11.1.9
  • gastrointestinal glutathione peroxidase 2
  • Gastrointestinal glutathione peroxidase
  • GIGPX
  • GI-GPx
  • glutathione peroxidase 2 (gastrointestinal)
  • Glutathione Peroxidase 2
  • Glutathione peroxidase-gastrointestinal
  • Glutathione peroxidase-related protein 2
  • GPRP
  • GPRP-2
  • GPX2
  • GPx-2
  • GPx-GI
  • GSHPx-2
  • GSHPX-GI

Background

This gene is a member of the glutathione peroxidase family and encodes a selenium-dependent glutathione peroxidase that is one of two isoenzymes responsible for the majority of the glutathione-dependent hydrogen peroxide-reducing activity in the epithelium of the gastrointestinal tract. Studies in knockout mice indicate that mRNA expression levels respond to luminal microflora, suggesting a role of the ileal glutathione peroxidases in preventing inflammation in the GI tract.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GPX2
Entrez
Uniprot