Home » CCL2/JE/MCP-1 » CCL2/JE/MCP-1 Antibodies » CCL2/JE/MCP-1 Antibody [Unconjugated]

CCL2/JE/MCP-1 Antibody [Unconjugated]

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Western blot shows 25 ng of Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE), Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE), Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME), Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5), and Recombinant Mouse CCL7/MCP-3/MARC (Catalog # 456-MC). PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse CCL2/JE/MCP-1 Polyclonal Antibody (Catalog # AB-479-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL2/JE/MCP-1 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Western blot shows 25 ng of Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE), Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE), Recombinant Mouse CCL11/Eotaxin ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

  • Size / Catalog#QtyPrice
    AB-479-NA
    1 mg
    $509.00
    1-3 Business Days
Shipping $45.00 per order
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CCL2/JE/MCP-1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse CCL2/JE/MCP-1
Gln24-Arg96
Accession # Q5SVU3
Specificity
Detects mouse CCL2/JE/MCP-1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human MCP-1, recombinant mouse (rm MCP-5, and recombinant rat JE is observed. In Western blots, no cross-reactivity with rmEotaxin, rmMCP-5, or rmCCL7 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
Ccl2
Purity Statement
Protein A or G purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
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Applications/Dilutions

Dilutions
  • Neutralization 10-30 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AB-479-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL2/JE/MCP-1 Antibody [Unconjugated]

  • C-C motif chemokine ligand 2
  • CCL2
  • GDCF-2
  • HC11
  • HSMCR30
  • MCAF
  • Mcp1
  • MCP-1
  • SCYA2
  • SMC-CF

Background

Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The murine CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in murine fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.

  1. Rollins, B.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3738.
  2. Gu, L. et al. (1999) Chem. Immunol. 72:7.
  3. Luini, W. et al. (1994) Cytokine 6:28.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Phone: 1-888-506-6887
Phone: 303-730-1950
Fax: 303-730-1966
Email: novus@novusbio.com
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Bioinformatics

Gene Symbol Ccl2
Uniprot