Western Blot: TREX1 Antibody [NBP1-76977] - Analysis of human spleen tissue lysate with TREX1 antibody at (A) 0.5 ug/mL and (B) 1 ug/mL.
Immunohistochemistry: TREX1 Antibody - BSA Free [NBP1-76977] - Immunohistochemistry of TREX1 in human spleen tissue with TREX1 antibody at 2.5 u/mL.
Immunocytochemistry/ Immunofluorescence: TREX1 Antibody - BSA Free [NBP1-76977] - Immunofluorescence of TREX1 in Human Spleen tissue with TREX1 antibody at 20 ug/mL.
Antibody was raised against a 15 amino acid synthetic peptide near the center of human TREX1. The immunogen is located within amino acids 220 - 270 of TREX1. Amino Acid Squence: TRLYGQSPPDSHT
Specificity
TREX1 antibody will not cross-react with the related protein TREX2. At least three isoforms of TREX1 are known to exist; this antibody will recognize all three isoforms.
Predicted Species
Bovine (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TREX1
Purity
Peptide affinity purified
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31 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified
Alternate Names for TREX1 Antibody - BSA Free
3'-5' exonuclease TREX1
AGS1
Aicardi-Goutieres syndrome 1
CRV
DKFZp434J0310
DNase III
DRN3
EC 3.1.11.2
HERNS
three prime repair exonuclease 1,3' repair exonuclease 1
Background
Trex1 is the major human 3' to 5' exonuclease which is required for checkpoint signaling after DNA damage. It is ubiquitously expressed, binds to single stranded DNA coated with replication protein A that accumulates at sites of DNA damage and recruits the ataxia telangiectasia and Rad3 related protein (ATR), a checkpoint kinase, to sites of DNA damage and replication stress. Trex1 is required for ATR expression. This gene uses two different open reading frames. The upstream ORF encodes proteins which interact with ATR and localize to intranuclear foci induced by DNA damage and are essential components of the DNA damage checkpoint. The downstream ORF encodes proteins with 3' to 5' exonuclease activity and may be a subunit of human DNA polymerase III. Multiple transcript variants encoding different isoforms have been found. Mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, and Cree encephalitis. TREX1 antibody will not cross-react with the related protein TREX2.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Dinosaur Protein Names: Infographic Trex1 the protein is involved in DNA damage response. Tyrannosaurus rex (T. rex) the dinosaur lived during the Cretaceous Period. Raptor the protein is a regulator of mTOR activity. Velociraptors the dinosaurs lived during the Cretaceous Period. Learn... Read full blog post.
Trex1 (3'-5' exonuclease TREX1, DNase III) This gene encodes the major 3' to 5' DNA exonuclease in human cells. The protein is a non-processive exonuclease that appears to provide proofreading for checkpoint signaling after DNA damage in response to oxidative stress and apoptosis. It is ubiqui... Read full blog post.
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