ABCA4 Antibody (3F4): sc-65672

ABCA4 Antibody (3F4) is a mouse monoclonal IgG1 kappa light chain antibody that detects ABCA4 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-ABCA4 antibody (3F4) is available in both conjugated and non-conjugated forms, providing versatility for various experimental needs. ABCA4, a member of the ATP-binding cassette (ABC) superfamily, plays a crucial role in the transport of essential molecules across photoreceptor cell membranes, which are vital for vision. ABCA4 is responsible for the transport of all-trans-retinal, a key component in the visual cycle, thereby preventing the accumulation of toxic byproducts that can lead to photoreceptor cell damage. Exclusive expression of ABCA4 in photoreceptor cells highlights importance in maintaining retinal health and function. Mutations in ABCA4 are linked to several retinal diseases, including autosomal recessive Stargardt disease, which is characterized by early-onset macular degeneration, as well as various forms of cone-rod dystrophy and retinitis pigmentosa. Individuals who are heterozygous for ABCA4 mutations may have an increased risk of developing age-related macular degeneration. ABCA4 is closely related to mouse and human ABC1 and ABC2 proteins and is located on human chromosome 1p22.1, underscoring evolutionary significance and potential as a therapeutic target in retinal diseases.

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ABCA4 Antibody (3F4) References:

1. Biochemical defects in ABCR protein variants associated with human retinopathies.  |  Sun, H., et al. 2000. Nat Genet. 26: 242-6. PMID: 11017087
2. Late-onset Stargardt disease is associated with missense mutations that map outside known functional regions of ABCR (ABCA4).  |  Yatsenko, AN., et al. 2001. Hum Genet. 108: 346-55. PMID: 11379881
3. Spectrum of ABCA4 (ABCR) gene mutations in Spanish patients with autosomal recessive macular dystrophies.  |  Paloma, E., et al. 2001. Hum Mutat. 17: 504-10. PMID: 11385708
4. Structural model of ATP-binding proteins associated with cystic fibrosis, multidrug resistance and bacterial transport.  |  Hyde, SC., et al. 1990. Nature. 346: 362-5. PMID: 1973824
5. Mutation Spectrum of the ABCA4 Gene in 335 Stargardt Disease Patients From a Multicenter German Cohort-Impact of Selected Deep Intronic Variants and Common SNPs.  |  Schulz, HL., et al. 2017. Invest Ophthalmol Vis Sci. 58: 394-403. PMID: 28118664
6. Clinical spectrum, genetic complexity and therapeutic approaches for retinal disease caused by ABCA4 mutations.  |  Cremers, FPM., et al. 2020. Prog Retin Eye Res. 79: 100861. PMID: 32278709
7. Resolving the dark matter of ABCA4 for 1054 Stargardt disease probands through integrated genomics and transcriptomics.  |  Khan, M., et al. 2020. Genet Med. 22: 1235-1246. PMID: 32307445
8. Clinical and genetic analysis of the ABCA4 gene associated retinal dystrophy in a large Chinese cohort.  |  Sun, Z., et al. 2021. Exp Eye Res. 202: 108389. PMID: 33301772
9. Structure and function of ABCA4 and its role in the visual cycle and Stargardt macular degeneration.  |  Molday, RS., et al. 2022. Prog Retin Eye Res. 89: 101036. PMID: 34954332
10. Evolution of ATP-binding cassette transporter genes.  |  Dean, M. and Allikmets, R. 1995. Curr Opin Genet Dev. 5: 779-85. PMID: 8745077
11. Mutation of the Stargardt disease gene (ABCR) in age-related macular degeneration.  |  Allikmets, R., et al. 1997. Science. 277: 1805-7. PMID: 9295268
12. ABC transporter-facilitated ATP conductive transport.  |  Schwiebert, EM. 1999. Am J Physiol. 276: C1-8. PMID: 9886914

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ABCA4 Antibody (3F4) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-ABCA4 Antibody (3F4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ABCA4 Antibody (3F4) Specifications

Alternate Product Name: ABCA4

Product Clone Name: 3F4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: ABCA4

Epitope Species: bovine

Product Reactivity/Detection: ABCA4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for ABCA4 Antibody (3F4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, PE: Phycoerythrin