Western Blot: ERCC6L Antibody [H00054821-D01P] - Analysis of ERCC6L expression in human colon.
Immunocytochemistry/ Immunofluorescence: ERCC6L Antibody [H00054821-D01P] - IF analysis of ERCC6L in mouse and human cell lines. Image courtesy of product review submitted by Corentin Laulier.
Western Blot: ERCC6L Antibody [H00054821-D01P] - Analysis of ERCC6L expression in transfected 293T cell line by ERCC6L polyclonal antibody.Lane 1: ERCC6L transfected lysate(46.20 KDa).Lane 2: Non-transfected lysate.
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. This antibody may also be useful in Immunofluorescence, as per a customer product review. Immunocytochemistry/Immunofluorescence was reported in scientific literature. Use in Immunohistochemistry reported in scientific literature (PMID 24589582)
Reviewed Applications
Read 1 Review rated 5 using H00054821-D01P in the following applications:
PICHexcision repair cross-complementing rodent repair deficiency complementationgroup 6 - like
PLK1-interacting checkpoint helicase
SNF2/RAD54 family protein
Tumor antigen BJ-HCC-15
Background
DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: ERCC6L Antibody [H00054821-D01P] - Analysis of ERCC6L expression in human colon.](http://images.novusbio.com/fullsize/ERCC6L-Antibody-Western-Blot-H00054821-D01P-img0004.jpg "Western Blot: ERCC6L Antibody [H00054821-D01P] - Analysis of ERCC6L expression in human colon.")
![Immunocytochemistry/Immunofluorescence: ERCC6L Antibody [H00054821-D01P] - IF analysis of ERCC6L in mouse and human cell lines. Image courtesy of product review submitted by Corentin Laulier.](http://images.novusbio.com/fullsize/ERCC6L-Antibody-Immunocytochemistry-Immunofluorescence-H00054821-D01P-img0002.jpg "Immunocytochemistry/Immunofluorescence: ERCC6L Antibody [H00054821-D01P] - IF analysis of ERCC6L in mouse and human cell lines. Image courtesy of product review submitted by Corentin Laulier.")
![Western Blot: ERCC6L Antibody [H00054821-D01P] - Analysis of ERCC6L expression in transfected 293T cell line by ERCC6L polyclonal antibody.Lane 1: ERCC6L transfected lysate(46.20 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ERCC6L-Antibody-Western-Blot-H00054821-D01P-img0005.jpg "Western Blot: ERCC6L Antibody [H00054821-D01P] - Analysis of ERCC6L expression in transfected 293T cell line by ERCC6L polyclonal antibody.Lane 1: ERCC6L transfected lysate(46.20 KDa).Lane 2: Non-transfected lysate.")
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![Simple Western Glutathione Peroxidase 1/GPX1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Glutathione_Peroxidase_1_AF3798_Simple_Western_17131.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
