SPRED1 Antibody (E-5): sc-393198
- SPRED1 Antibody (E-5) is a mouse monoclonal IgG1 κ SPRED1 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 135-246 mapping within an internal region of SPRED1 of human origin
- SPRED1 Antibody (E-5) is recommended for detection of SPRED1 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-SPRED1 Antibody (E-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SPRED1 (E-5): sc-393198.
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SPRED1 Antibody (E-5) for WB and IHC(P) applications. These reagents are now offered in bundles with SPRED1 Antibody (E-5) (see ordering information below).
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SPRED1 Antibody (E-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects SPRED1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). SPRED1 (E-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SPRED1, also known as sprouty-related, EVH1 domain containing 1 or NFLS, is a 444 amino acid protein characterized by the presence of one KBD domain, one sprouty domain, and one WH1 domain. SPRED1 is primarily localized to the cell membrane and cholesterol-rich membrane raft fractions, where SPRED1 plays a crucial role in regulating the activity of the extracellular signal-regulated kinase (ERK) cascade by inhibiting growth-factor-mediated activation of ERK. The regulation of ERK is vital for controlling cell proliferation and differentiation, making SPRED1 an important player in cellular signaling pathways. Additionally, SPRED1 can function as a homodimer or as a heterodimer with SPRED2, and SPRED1 is believed to negatively regulate the development of blood cells in the bone marrow. Mutations in the gene encoding SPRED1 are associated with neurofibromatosis type 1-like syndrome (NFLS), an autosomal dominant disorder characterized by multiple café-au-lait spots, axillary freckling, and macrocephaly, highlighting the significance of SPRED1 in both normal physiology and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SPRED1 Antibody (E-5) References:
- Identification of SPRED1 deletions using RT-PCR, multiplex ligation-dependent probe amplification and quantitative PCR. | Spencer, E., et al. 2011. Am J Med Genet A. 155A: 1352-9. PMID: 21548021
- SPRED1, a RAS MAPK pathway inhibitor that causes Legius syndrome, is a tumour suppressor downregulated in paediatric acute myeloblastic leukaemia. | Pasmant, E., et al. 2015. Oncogene. 34: 631-8. PMID: 24469042
- The neurofibromin recruitment factor Spred1 binds to the GAP related domain without affecting Ras inactivation. | Dunzendorfer-Matt, T., et al. 2016. Proc Natl Acad Sci U S A. 113: 7497-502. PMID: 27313208
- SPRED1 Interferes with K-ras but Not H-ras Membrane Anchorage and Signaling. | Siljamäki, E. and Abankwa, D. 2016. Mol Cell Biol. 36: 2612-25. PMID: 27503857
- Spred1 Safeguards Hematopoietic Homeostasis against Diet-Induced Systemic Stress. | Tadokoro, Y., et al. 2018. Cell Stem Cell. 22: 713-725.e8. PMID: 29706577
- Role of SPRED1 in keratinocyte proliferation in psoriasis. | Li, X., et al. 2020. J Dermatol. 47: 735-742. PMID: 32396270
- SPRED1 deletion confers resistance to MAPK inhibition in melanoma. | Ablain, J., et al. 2021. J Exp Med. 218: PMID: 33306107
- Impaired instrumental learning in Spred1-/- mice, a model for a rare RASopathy. | Borrie, SC., et al. 2021. Genes Brain Behav. 20: e12727. PMID: 33624414
- SPOCK2 and SPRED1 function downstream of EZH2 to impede the malignant progression of lung adenocarcinoma in vitro and in vivo. | Liu, Y., et al. 2023. Hum Cell. 36: 812-821. PMID: 36629984
- Circ_TEX2 Functions as a Tumor Suppressor in Hepatoma via miR-96-5p/SPRED1 Axis. | Yuan, Q., et al. 2023. Mol Biotechnol. 65: 1679-1692. PMID: 36745282
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPRED1 Antibody (E-5) | sc-393198 | 200 µg/ml | $316.00 | |||
SPRED1 Antibody (E-5): m-IgGκ BP-HRP Bundle | sc-523847 | 200 µg Ab, 40 µg BP | $354.00 | |||
SPRED1 Antibody (E-5): m-IgG1 BP-HRP Bundle | sc-533613 | 200 µg Ab; 20 µg BP | $354.00 | |||
SPRED1 Antibody (E-5) AC | sc-393198 AC | 500 µg/ml, 25% agarose | $416.00 | |||
SPRED1 Antibody (E-5) HRP | sc-393198 HRP | 200 µg/ml | $316.00 | |||
SPRED1 Antibody (E-5) FITC | sc-393198 FITC | 200 µg/ml | $330.00 | |||
SPRED1 Antibody (E-5) PE | sc-393198 PE | 200 µg/ml | $343.00 | |||
SPRED1 Antibody (E-5) Alexa Fluor® 488 | sc-393198 AF488 | 200 µg/ml | $357.00 | |||
SPRED1 Antibody (E-5) Alexa Fluor® 546 | sc-393198 AF546 | 200 µg/ml | $357.00 | |||
SPRED1 Antibody (E-5) Alexa Fluor® 594 | sc-393198 AF594 | 200 µg/ml | $357.00 | |||
SPRED1 Antibody (E-5) Alexa Fluor® 647 | sc-393198 AF647 | 200 µg/ml | $357.00 | |||
SPRED1 Antibody (E-5) Alexa Fluor® 680 | sc-393198 AF680 | 200 µg/ml | $357.00 | |||
SPRED1 Antibody (E-5) Alexa Fluor® 790 | sc-393198 AF790 | 200 µg/ml | $357.00 |