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PRX Antibody (B-11): sc-137222

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Datasheets
  • PRX Antibody (B-11) is a mouse monoclonal IgG1 κ PRX antibody, cited in 6 publications, provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 123-161 near the N-terminus of PRX II of human origin
  • PRX Antibody (B-11) is recommended for detection of PRX I, PRX II and PRX IV of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine and porcine
  • Anti-PRX Antibody (B-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PRX (B-11): sc-137222.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PRX Antibody (B-11) for WB and IHC(P) applications. These reagents are now offered in bundles with PRX Antibody (B-11) (see ordering information below).

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PRX Antibody (B-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects PRX protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PRX (B-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The peroxiredoxin (PRX) family consists of six antioxidant proteins—PRX I, II, III, IV, V, and VI—that play a crucial role in protecting cells from reactive oxygen species (ROS) by preventing metal-catalyzed oxidation of enzymes. PRX proteins primarily utilize thioredoxin as their electron donor for antioxidation, although PRX proteins can interact with various hydroperoxide substrates. Peroxiredoxins are implicated in essential cellular processes such as proliferation, differentiation, and gene expression. PRX I, II, IV, and VI are found diffusely in the cytoplasm, while PRX III and V are specifically localized to mitochondria, highlighting distinct roles in cellular metabolism and signaling. PRX I is expressed across various tissues, including liver, kidney, testis, lung, and nervous system, and PRX I overexpression has been linked to breast cancer, suggesting a potential role in tumorigenesis. Elevated levels of PRX I and II have been observed in Alzheimer′s disease and Down syndrome, indicating involvement in pathogenesis of these conditions. PRX IV is notably expressed in many tissues, existing in both a precursor form detected only in testis and a processed secreted form, while PRX V has long and short forms, with long form predominantly expressed in testis and short form found in liver, kidney, heart, and lung. PRX VI, also known as antioxidant protein 2, is highly expressed in epithelial cells and functions independently of other peroxiredoxins, specializing in antioxidant defense, lung phospholipid metabolism, and protecting keratinocytes from ROS-induced cell death.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

PRX Antibody (B-11) References:

  1. Peroxiredoxin I (macrophage 23 kDa stress protein) is highly and widely expressed in the rat nervous system.  |  Mizusawa, H., et al. 2000. Neurosci Lett. 283: 57-60. PMID: 10729633
  2. From cytoprotection to tumor suppression: the multifactorial role of peroxiredoxins.  |  Butterfield, LH., et al. 1999. Antioxid Redox Signal. 1: 385-402. PMID: 11233141
  3. Overexpression of peroxiredoxin in human breast cancer.  |  Noh, DY., et al. 2001. Anticancer Res. 21: 2085-90. PMID: 11497302
  4. Protein levels of human peroxiredoxin subtypes in brains of patients with Alzheimer's disease and Down syndrome.  |  Kim, SH., et al. 2001. J Neural Transm Suppl. 223-35. PMID: 11771746
  5. Cell specific expression of peroxiredoxins in human lung and pulmonary sarcoidosis.  |  Kinnula, VL., et al. 2002. Thorax. 57: 157-64. PMID: 11828047
  6. Peroxiredoxins.  |  Hofmann, B., et al. 2002. Biol Chem. 383: 347-64. PMID: 12033427
  7. Advances in our understanding of peroxiredoxin, a multifunctional, mammalian redox protein.  |  Fujii, J. and Ikeda, Y. 2002. Redox Rep. 7: 123-30. PMID: 12189041
  8. Mice with targeted mutation of peroxiredoxin 6 develop normally but are susceptible to oxidative stress.  |  Wang, X., et al. 2003. J Biol Chem. 278: 25179-90. PMID: 12732627
  9. Purification, characterization, and cloning of a heme-binding protein (23 kDa) in rat liver cytosol.  |  Iwahara, S., et al. 1995. Biochemistry. 34: 13398-406. PMID: 7577926

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

PRX Antibody (B-11)

sc-137222
200 µg/ml
$316.00

PRX Antibody (B-11): m-IgG Fc BP-HRP Bundle

sc-528878
200 µg Ab; 10 µg BP
$354.00

PRX Antibody (B-11): m-IgGκ BP-HRP Bundle

sc-521457
200 µg Ab, 40 µg BP
$354.00

PRX Antibody (B-11): m-IgG1 BP-HRP Bundle

sc-543187
200 µg Ab; 20 µg BP
$354.00

PRX Antibody (B-11) AC

sc-137222 AC
500 µg/ml, 25% agarose
$416.00

PRX Antibody (B-11) HRP

sc-137222 HRP
200 µg/ml
$316.00

PRX Antibody (B-11) FITC

sc-137222 FITC
200 µg/ml
$330.00

PRX Antibody (B-11) PE

sc-137222 PE
200 µg/ml
$343.00

PRX Antibody (B-11) Alexa Fluor® 488

sc-137222 AF488
200 µg/ml
$357.00

PRX Antibody (B-11) Alexa Fluor® 546

sc-137222 AF546
200 µg/ml
$357.00

PRX Antibody (B-11) Alexa Fluor® 594

sc-137222 AF594
200 µg/ml
$357.00

PRX Antibody (B-11) Alexa Fluor® 647

sc-137222 AF647
200 µg/ml
$357.00

PRX Antibody (B-11) Alexa Fluor® 680

sc-137222 AF680
200 µg/ml
$357.00

PRX Antibody (B-11) Alexa Fluor® 790

sc-137222 AF790
200 µg/ml
$357.00

PRX (B-11) Neutralizing Peptide

sc-137222 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-137222 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from SC-137222The antibody performs high sensitivity and specificity.
Date published: 2020-08-31
Rated 5 out of 5 by from Good for WBWorks very well on HeLa cell extract. Dilution 1:500. You can see a strong band at 25 kDa
Date published: 2018-10-10
Rated 5 out of 5 by from Great antibody!1:500 diluted for wb of rat protein,the band is strong and special!
Date published: 2018-02-13
Rated 5 out of 5 by from GOOD BY WBIT WORKED WELL BY WB IN LAB, WILL PURCHASE IT IN THE FUTURE
Date published: 2017-02-20
Rated 5 out of 5 by from An antioxidantWe specifically use thioredoxin as an antioxidant electron donor, although there are still magazines. But still effective protection of peroxide also involved in cell proliferation and differentiation.
Date published: 2017-02-10
Rated 5 out of 5 by from Strong band seen in MCF7 samplesStrong band seen in MCF7 samples. -SCBT QC
Date published: 2015-01-09
Rated 5 out of 5 by from WB publishable data with pig ventricular tissueWB publishable data with pig ventricular tissue - SCBT Publication Review
Date published: 2013-04-19
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PRX Antibody (B-11) is rated 5.0 out of 5 by 7.
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