MTHFD2 Antibody (A-2): sc-390708

MTHFD2 Antibody (A-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects MTHFD2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-MTHFD2 antibody (A-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MTHFD2, or Methylenetetrahydrofolate dehydrogenase 2, is a crucial 350 amino acid bifunctional enzyme that plays a vital role in the interconversion of tetrahydrofolate derivatives, which are essential for the synthesis of purine, methionine, and thymidylate. MTHFD2 exhibits both methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities, requiring either NADP or NAD as cofactors for enzymatic functions. MTHFD2 activity is significantly influenced by intracellular concentrations of magnesium and phosphate, which are critical for maintaining cellular metabolism. MTHFD2 functions as a homodimer and is predominantly localized in the mitochondria, where MTHFD2 is expressed during the development of normal tissues, underscoring its importance in cellular energy metabolism and overall cellular health.

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MTHFD2 Antibody (A-2) References:

1. Mitochondrial NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase is essential for embryonic development.  |  Di Pietro, E., et al. 2002. Mol Cell Biol. 22: 4158-66. PMID: 12024029
2. NAD- and NADP-dependent mitochondrially targeted methylenetetrahydrofolate dehydrogenase-cyclohydrolases can rescue mthfd2 null fibroblasts.  |  Patel, H., et al. 2005. Arch Biochem Biophys. 442: 133-9. PMID: 16150419
3. Transpupillary thermotherapy-induced modification of angiogenesis- and coagulation-related gene expression in the rat posterior fundus.  |  Ito, YN., et al. 2006. Mol Vis. 12: 802-10. PMID: 16885923
4. Expression profiling of PC-3 cell line variants and comparison of MIC-1 transcript levels in benign and malignant prostate.  |  Patrikainen, L., et al. 2007. Eur J Clin Invest. 37: 126-33. PMID: 17217378
5. Cloning of three human multifunctional de novo purine biosynthetic genes by functional complementation of yeast mutations.  |  Schild, D., et al. 1990. Proc Natl Acad Sci U S A. 87: 2916-20. PMID: 2183217
6. Nucleotide sequence of the human NAD-dependent methylene tetrahydrofolate dehydrogenase-cyclohydrolase.  |  Peri, KG., et al. 1989. Nucleic Acids Res. 17: 8853. PMID: 2587219
7. Pharmacological targeting of MTHFD2 suppresses acute myeloid leukemia by inducing thymidine depletion and replication stress.  |  Bonagas, N., et al. 2022. Nat Cancer. 3: 156-172. PMID: 35228749
8. Purification and characterization of a mitochondrial isozyme of C1-tetrahydrofolate synthase from Saccharomyces cerevisiae.  |  Shannon, KW. and Rabinowitz, JC. 1986. J Biol Chem. 261: 12266-71. PMID: 3528153
9. Folate enzyme MTHFD2 links one-carbon metabolism to unfolded protein response in glioblastoma.  |  Zhu, Z., et al. 2022. Cancer Lett. 549: 215903. PMID: 36089117
10. MTHFD2 ablation in T cells protects against heart transplant rejection by perturbing IRF4/PD-1 pathway through the metabolic-epigenetic nexus.  |  Li, Y., et al. 2023. J Heart Lung Transplant. 42: 1608-1620. PMID: 37495036
11. The one-carbon metabolic enzyme MTHFD2 promotes resection and homologous recombination after ionizing radiation.  |  Marttila, P., et al. 2024. Mol Oncol. 18: 2179-2195. PMID: 38533616
12. NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase is the mammalian homolog of the mitochondrial enzyme encoded by the yeast MIS1 gene.  |  Yang, XM. and MacKenzie, RE. 1993. Biochemistry. 32: 11118-23. PMID: 8218174

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MTHFD2 Antibody (A-2) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-MTHFD2 Antibody (A-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MTHFD2 Antibody (A-2) Specifications

Alternate Product Name: MTHFD2

Product Clone Name: A-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 197-350

Epitope Target: MTHFD2

Epitope Species: human

Product Reactivity/Detection: MTHFD2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for MTHFD2 Antibody (A-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin