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western blotting image from recommended MRP1 (QCRL-1) monoclonal antibody replacement. Image 379308
This polyclonal antibody has been discontinued. See our recommended MRP1 (QCRL-1) monoclonal antibody (sample data shown).
western blotting image from recommended MRP1 (QCRL-1) monoclonal antibody replacement. Image 379308
This polyclonal antibody has been discontinued. See our recommended MRP1 (QCRL-1) monoclonal antibody (sample data shown).
This polyclonal antibody has been discontinued. See our recommended MRP1 (QCRL-1) monoclonal antibody (sample data shown).

MRP1 Antibody (H-70): sc-13960

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  • MRP1 Antibody (H-70) is a rabbit polyclonal IgG; 200 µg/ml
  • epitope corresponding to amino acids 1-70 mapping at the N-terminus of MRP1 of human origin
  • Discontinued polyclonal antibody

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Description

SEE ALSO...

Additional MRP Antibodies including MRP2, MRP3, MRP4, MRP5 and MRP6

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

Ordering Information

MRP1 (QCRL-1): sc-18835 [ Recommended monoclonal replacement for MRP1 (H-70) ]

Product NameCatalog #UNITPriceQtyFAVORITES

MRP1 Antibody (QCRL-1)

sc-18835
200 µg/ml
$316.00

MRP1 Antibody (QCRL-1): m-IgG Fc BP-HRP Bundle

sc-528336
200 µg Ab; 10 µg BP
$354.00

MRP1 Antibody (QCRL-1): m-IgGκ BP-HRP Bundle

sc-520688
200 µg Ab, 40 µg BP
$354.00

MRP1 Antibody (QCRL-1): m-IgG1 BP-HRP Bundle

sc-542874
200 µg Ab; 20 µg BP
$354.00

MRP1 Antibody (QCRL-1) AC

sc-18835 AC
500 µg/ml, 25% agarose
$416.00

MRP1 Antibody (QCRL-1) HRP

sc-18835 HRP
200 µg/ml
$316.00

MRP1 Antibody (QCRL-1) FITC

sc-18835 FITC
200 µg/ml
$330.00

MRP1 Antibody (QCRL-1) PE

sc-18835 PE
200 µg/ml
$343.00

MRP1 Antibody (QCRL-1) Alexa Fluor® 488

sc-18835 AF488
200 µg/ml
$357.00

MRP1 Antibody (QCRL-1) Alexa Fluor® 546

sc-18835 AF546
200 µg/ml
$357.00

MRP1 Antibody (QCRL-1) Alexa Fluor® 594

sc-18835 AF594
200 µg/ml
$357.00

MRP1 Antibody (QCRL-1) Alexa Fluor® 647

sc-18835 AF647
200 µg/ml
$357.00

MRP1 Antibody (QCRL-1) Alexa Fluor® 680

sc-18835 AF680
200 µg/ml
$357.00

MRP1 Antibody (QCRL-1) Alexa Fluor® 790

sc-18835 AF790
200 µg/ml
$357.00

I couldn't get any good immunoblot even after loading 80 ug of protein on a 8% SDS PAGE with Biorad nitrocellulose membrane. Very faint or ghost band are visible. I am using RIPA buffer for lysis and I work on MSCs. Any help will be greatly appreciated.

Asked by: Haroon
Thank you for your question. We have not tested this antibody on MSC's, and do not know how abundant MRP1 is in that sample. We recommend using T98G, A549 or AML-193 whole cell lysates as positive controls with our MRP1 antibodies. We recommend loading 50 µg protein, and using the primary antibody at a dilution of 1:200. A link to our complete recommended protocol for Western blotting is provided below. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting This antibody, MRP1 (H-70): sc-13960, is a rabbit polyclonal that is now discontinued. We would highly recommend a replacement mouse monoclonal, MRP1 Antibody (QCRL-1): sc-18835 (https://www.scbt.com/scbt/product/mrp1-antibody-qcrl-1). Please contact our Technical Service Department for more information.
Answered by: Technical Support
Date published: 2017-02-01
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Rated 4 out of 5 by from WB data has been showing good resultsWB data has been showing good results. We are still testing with it in lab.
Date published: 2015-09-17
Rated 5 out of 5 by from This antibody has been working well in our WBThis antibody has been working well in our WB.
Date published: 2015-09-04
Rated 5 out of 5 by from Published data for WB in human CacoPublished data for WB in human Caco-2 cells. -SCBT Publication Review
Date published: 2015-05-18
Rated 5 out of 5 by from Published data for WB in human ACHN and SN12K1Published data for WB in human ACHN and SN12K1 cells. -SCBT Publication Review
Date published: 2015-04-26
Rated 5 out of 5 by from Great immunoperoxidase membrane and cytoplasmicGreat immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human testis tissue. -SCBT QC
Date published: 2015-04-11
Rated 5 out of 5 by from Published IHC data for use in rat nasal respiratoryPublished IHC data for use in rat nasal respiratory mucosa , olfactory mucosa , OB, liver, and kidney. -SCBT Publication Review
Date published: 2015-03-11
Rated 5 out of 5 by from Published data for IP and WB in rat lungPublished data for IP and WB in rat lung. -SCBT Publication Review
Date published: 2015-01-24
Rated 5 out of 5 by from Published data for IF(pfa) in human HTRPublished data for IF(pfa) in human HTR-8/SVneo and JAR cells. -SCBT Publication Review
Date published: 2015-01-06
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MRP1 Antibody (H-70) is rated 4.9 out of 5 by 8.
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