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Enolase Antibody (B-8): sc-271792

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Datasheets
  • Enolase Antibody (B-8) is a mouse monoclonal IgM κ, cited in 1 publications, provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 391-413 near the C-terminus of α Enolase of human origin
  • recommended for detection of α Enolase, β Enolase and γ Enolase of mouse, rat, human and avian origin by WB, IP, IF and ELISA
  • See Enolase (A-5): sc-271384 for Enolase antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Enolase Antibody (B-8). This work is in progress.

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Enolase Antibody (B-8) is a mouse monoclonal IgM antibody that detects Enolase in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Enolase is a crucial enzyme in the glycolytic pathway, facilitating the conversion of 2-phosphoglycerate to phosphoenolpyruvate, a key step in energy production. This process is vital for cellular metabolism, particularly in tissues with high energy demands, such as muscle and brain. The enzyme exists in three isoforms—α, β, and γ—each with distinct tissue distribution and functional roles, highlighting the importance of enolase in various physiological contexts. Notably, α Enolase is expressed widely across tissues, while β Enolase is predominantly found in muscle, and γ Enolase is specific to nervous tissue. Enolase consists of 433 amino acids and exhibits significant evolutionary conservation, showing 67% homology to yeast enolase and 94% homology to rat nonneural enolase. Additionally, α Enolase is encoded by the same gene as tau-crystallin, a structural protein in the lens, underscoring its multifaceted roles in both metabolic and structural functions within cells. Anti-Enolase antibody (B-8) is an essential tool for researchers studying glycolysis and its implications in various biological processes and diseases.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Enolase Antibody (B-8)

sc-271792
200 µg/ml
$316.00

Enolase (B-8) Neutralizing Peptide

sc-271792 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-271792 P appropriate for?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
  • y_2025, m_3, d_31, h_7CST
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Rated 5 out of 5 by from Positive signal in WB in human transfectedPositive signal in WB in human transfected 293 whole cell lysates. -SCBT QC
Date published: 2015-04-22
Rated 5 out of 5 by from Strong band in WB in Y79 whole cell lysateStrong band in WB in Y79 whole cell lysate. -SCBT QC
Date published: 2015-03-02
Rated 5 out of 5 by from WB publishable data with rat pancreatic acinarWB publishable data with rat pancreatic acinar AR42J cells - SCBT Publication Review
Date published: 2015-02-04
Rated 5 out of 5 by from Beautiful immunofluorescence cytoplasmicBeautiful immunofluorescence cytoplasmic staining in methanol-fixed NIH/3T3 cells. -SCBT QC
Date published: 2013-05-02
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Enolase Antibody (B-8) is rated 5.0 out of 5 by 4.
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