DSPP Antibody (LFMb-21): sc-73632

DSPP Antibody (LFMb-21) is a mouse monoclonal IgG2b kappa light chain antibody that detects DSPP protein of mouse, rat, human, and monkey origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. DSPP (LFMb-21) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DSPP protein, or dentin sialophosphoprotein, serves as a precursor that is cleaved into two functional proteins: dentin sialoprotein (DSP) and dentin phosphoprotein (DPP). These proteins are integral components of the dentin extracellular matrix in teeth, with DSP playing a crucial role in dentinogenesis, while DPP is essential for binding calcium, which facilitates the initial mineralization of the dentin matrix collagen and regulates the size and shape of mineral crystals. Proper functioning of DSPP is vital for tooth development and integrity, as mutations in the DSPP gene can lead to conditions such as DFNA39/DGI1, characterized by progressive hearing loss and dentinogenesis imperfecta, as well as DGI2 and DGI3, which result in brittle, discolored teeth. Understanding the role of DSPP and its derivatives in dental health is critical for developing therapeutic strategies for these genetic disorders.

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DSPP Antibody (LFMb-21) References:

1. Tissue-specific expression of dentin sialophosphoprotein (DSPP) and its polymorphisms in mouse tissues.  |  Yuan, G., et al. 2009. Cell Biol Int. 33: 816-29. PMID: 19450697
2. Enamel malformations associated with a defined dentin sialophosphoprotein mutation in two families.  |  Wang, SK., et al. 2011. Eur J Oral Sci. 119 Suppl 1: 158-67. PMID: 22243242
3. Mutation identification of the DSPP in a Chinese family with DGI-II and an up-to-date bioinformatic analysis.  |  Li, D., et al. 2012. Genomics. 99: 220-6. PMID: 22310900
4. Rough endoplasmic reticulum trafficking errors by different classes of mutant dentin sialophosphoprotein (DSPP) cause dominant negative effects in both dentinogenesis imperfecta and dentin dysplasia by entrapping normal DSPP.  |  von Marschall, Z., et al. 2012. J Bone Miner Res. 27: 1309-21. PMID: 22392858
5. Hereditary dentine diseases resulting from mutations in DSPP gene.  |  Maciejewska, I. and Chomik, E. 2012. J Dent. 40: 542-8. PMID: 22521702
6. Dentin sialophosphoprotein and dentin matrix protein-1: Two highly phosphorylated proteins in mineralized tissues.  |  Suzuki, S., et al. 2012. Arch Oral Biol. 57: 1165-75. PMID: 22534175
7. The efficiency of dentin sialoprotein-phosphophoryn processing is affected by mutations both flanking and distant from the cleavage site.  |  Yang, RT., et al. 2013. J Biol Chem. 288: 6024-33. PMID: 23297400
8. A DSPP mutation causing dentinogenesis imperfecta and characterization of the mutational effect.  |  Lee, SK., et al. 2013. Biomed Res Int. 2013: 948181. PMID: 23509818
9. Domain of dentine sialoprotein mediates proliferation and differentiation of human periodontal ligament stem cells.  |  Ozer, A., et al. 2013. PLoS One. 8: e81655. PMID: 24400037
10. Adhesive and migratory effects of phosphophoryn are modulated by flanking peptides of the integrin binding motif.  |  Suzuki, S., et al. 2014. PLoS One. 9: e112490. PMID: 25396425
11. Dentin sialophosphoprotein-derived proteins in porcine pulp and dentin - Gene expression and function.  |  Yamamoto, R. and Yamakoshi, Y. 2016. J Oral Biosci. 58: 120-127. PMID: 32512680
12. The role of Dentin Sialophosphoprotein (DSPP) in craniofacial development.  |  Figueredo, CA., et al. 2022. J Oral Biol Craniofac Res. 12: 673-678. PMID: 36062256

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DSPP Antibody (LFMb-21) has 115 citations in a variety of scientific publications.

Primary antibodies like Anti-DSPP Antibody (LFMb-21) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DSPP Antibody (LFMb-21) Specifications

Alternate Product Name: Dentin Sialophosphoprotein

Product Clone Name: LFMb-21

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 487-502

Epitope Target: DSPP

Epitope Species: human

Product Reactivity/Detection: DSPP and DPP

Species Reactivity/Detection: mouse, rat, human, monkey

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 115 citations in various scientific publications

Available Conjugates/Forms for DSPP Antibody (LFMb-21): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin