Dok-1 Antibody (45): sc-135888

Dok-1 Antibody (45) is a mouse monoclonal IgG1 antibody that detects Dok-1 in human samples through western blotting (WB) and immunoprecipitation (IP). Dok-1, also known as p62dok, plays a crucial role in cellular signaling pathways by acting as a docking protein that interacts with various tyrosine kinases, including the oncogenic p210 Bcr-Abl fusion protein associated with chronic myelogenous leukemia. Dok-1 is primarily located in the cytoplasm, where Dok-1 serves as a scaffold for assembling signaling complexes that regulate cell growth and differentiation. Proper localization of Dok-1 allows for efficient transmission of signals from growth factor receptors, such as those for platelet-derived growth factor (PDGF) and insulin-like growth factor (IGF), to downstream effectors. Dok-1 undergoes tyrosine phosphorylation in response to these growth factors, which enhances Dok-1′s ability to interact with other signaling molecules, thereby influencing cellular responses to external stimuli. Anti-Dok-1 antibody (45) is an essential tool for researchers studying the intricate signaling networks that govern cell behavior and the implications of their dysregulation in diseases such as cancer.

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Dok-1 Antibody (45) References:

1. Functional interaction of RasGAP-binding proteins Dok-1 and Dok-2 with the Tec protein tyrosine kinase.  |  Gérard, A., et al. 2004. Oncogene. 23: 1594-8. PMID: 14647425
2. Role of Dok-1 and Dok-2 in myeloid homeostasis and suppression of leukemia.  |  Yasuda, T., et al. 2004. J Exp Med. 200: 1681-7. PMID: 15611294
3. Dok-1 and Dok-2 are negative regulators of lipopolysaccharide-induced signaling.  |  Shinohara, H., et al. 2005. J Exp Med. 201: 333-9. PMID: 15699069
4. Dok-1 independently attenuates Ras/mitogen-activated protein kinase and Src/c-myc pathways to inhibit platelet-derived growth factor-induced mitogenesis.  |  Zhao, M., et al. 2006. Mol Cell Biol. 26: 2479-89. PMID: 16537894
5. Dok-1 and Dok-2 are negative regulators of T cell receptor signaling.  |  Yasuda, T., et al. 2007. Int Immunol. 19: 487-95. PMID: 17329234
6. Proteomic analysis of integrin alphaIIbbeta3 outside-in signaling reveals Src-kinase-independent phosphorylation of Dok-1 and Dok-3 leading to SHIP-1 interactions.  |  Senis, YA., et al. 2009. J Thromb Haemost. 7: 1718-26. PMID: 19682241
7. Mice lacking Dok-1, Dok-2, and Dok-3 succumb to aggressive histiocytic sarcoma.  |  Mashima, R., et al. 2010. Lab Invest. 90: 1357-64. PMID: 20548287
8. Loss of Dok-1 and Dok-2 in mice causes severe experimental colitis accompanied by reduced expression of IL-17A and IL-22.  |  Waseda, M., et al. 2016. Biochem Biophys Res Commun. 478: 135-142. PMID: 27450811
9. Dok-1 and Dok-2 Are Required To Maintain Herpes Simplex Virus 1-Specific CD8+ T Cells in a Murine Model of Ocular Infection.  |  Lahmidi, S., et al. 2017. J Virol. 91: PMID: 28490594
10. Dok-3 and Dok-1/-2 adaptors play distinctive roles in cell fusion and proliferation during osteoclastogenesis and cooperatively protect mice from osteopenia.  |  Kajikawa, S., et al. 2018. Biochem Biophys Res Commun. 498: 967-974. PMID: 29548825

The preferred Dok-1 antibody is Dok-1 Antibody (A-3): sc-6929.