CYP1A1 Antibody (B-4): sc-25304
- CYP1A1 Antibody (B-4) is a mouse monoclonal IgG2a κ, cited in 46 publications, provided at 200 µg/ml
- raised against amino acids 246-315 of CYP1A1 of human origin
- Anti-CYP1A1 Antibody (B-4) is recommended for detection of CYP1A1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-CYP1A1 Antibody (B-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CYP1A1 (B-4): sc-25304.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CYP1A1 Antibody (B-4). This work is in progress.
CYP1A1 Antibody (B-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects CYP1A1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-CYP1A1 antibody (B-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CYP1A1 is a member of the cytochrome P450 family of enzymes, which play a crucial role in metabolizing diverse endogenous compounds, such as hormones and fatty acids, as well as xenobiotics, including drugs and environmental toxins. Proper functioning of CYP1A1 is vital for detoxifying harmful substances and maintaining metabolic homeostasis, making CYP1A1 an important target for studying drug metabolism and toxicology. CYP1A1 is primarily located in the endoplasmic reticulum of liver cells, where CYP1A1 catalyzes substrate oxidation, highlighting CYP1A1′s significance in hepatic metabolism. Understanding CYP1A1 expression and regulation can provide insights into individual variations in drug response and susceptibility to toxic compounds, underscoring CYP1A1′s importance in pharmacogenomics and personalized medicine.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CYP1A1 Antibody (B-4) References:
- Structural and functional analysis of NADPH-cytochrome P-450 reductase from human liver: complete sequence of human enzyme and NADPH-binding sites. | Haniu, M., et al. 1989. Biochemistry. 28: 8639-45. PMID: 2513880
- Structural analysis of the FMN binding domain of NADPH-cytochrome P-450 oxidoreductase by site-directed mutagenesis. | Shen, AL., et al. 1989. J Biol Chem. 264: 7584-9. PMID: 2708380
- Human CYP1A1 inhibition by flavonoids. | Santes-Palacios, R., et al. 2020. Toxicol In Vitro. 62: 104681. PMID: 31655123
- The Activation of Procarcinogens by CYP1A1/1B1 and Related Chemo-Preventive Agents: A Review. | Li, Y., et al. 2021. Curr Cancer Drug Targets. 21: 21-54. PMID: 33023449
- CYP1A1 Enzymatic Activity Influences Skin Inflammation Via Regulation of the AHR Pathway. | Kyoreva, M., et al. 2021. J Invest Dermatol. 141: 1553-1563.e3. PMID: 33385398
- Inhibition of CYP1A1 Alleviates Colchicine-Induced Hepatotoxicity. | Huang, R., et al. 2024. Toxins (Basel). 16: PMID: 38251251
- Purified liver microsomal NADPH-cytochrome P-450 reductase. Spectral characterization of oxidation-reduction states. | Vermilion, JL. and Coon, MJ. 1978. J Biol Chem. 253: 2694-704. PMID: 632295
- Mouse NADPH-cytochrome P-450 oxidoreductase: molecular cloning and functional expression in yeast. | Ohgiya, S., et al. 1994. Biochim Biophys Acta. 1186: 137-41. PMID: 8011664
- NADPH-P-450 reductase: structural and functional comparisons of the eukaryotic and prokaryotic isoforms. | Sevrioukova, IF. and Peterson, JA. 1995. Biochimie. 77: 562-72. PMID: 8589067
- P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature. | Nelson, DR., et al. 1996. Pharmacogenetics. 6: 1-42. PMID: 8845856
- Quantitation of FAD-dependent cytochrome P450 reductase activity by photoreduction. | Hodgson, AV. and Strobel, HW. 1996. Anal Biochem. 243: 154-7. PMID: 8954538
- P450BM-3; a tale of two domains--or is it three? | Peterson, JA., et al. 1997. Steroids. 62: 117-23. PMID: 9029725
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CYP1A1 Antibody (B-4) | sc-25304 | 200 µg/ml | $316.00 | |||
CYP1A1 Antibody (B-4) AC | sc-25304 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CYP1A1 Antibody (B-4) HRP | sc-25304 HRP | 200 µg/ml | $316.00 | |||
CYP1A1 Antibody (B-4) FITC | sc-25304 FITC | 200 µg/ml | $330.00 | |||
CYP1A1 Antibody (B-4) PE | sc-25304 PE | 200 µg/ml | $343.00 | |||
CYP1A1 Antibody (B-4) Alexa Fluor® 488 | sc-25304 AF488 | 200 µg/ml | $357.00 | |||
CYP1A1 Antibody (B-4) Alexa Fluor® 546 | sc-25304 AF546 | 200 µg/ml | $357.00 | |||
CYP1A1 Antibody (B-4) Alexa Fluor® 594 | sc-25304 AF594 | 200 µg/ml | $357.00 | |||
CYP1A1 Antibody (B-4) Alexa Fluor® 647 | sc-25304 AF647 | 200 µg/ml | $357.00 | |||
CYP1A1 Antibody (B-4) Alexa Fluor® 680 | sc-25304 AF680 | 200 µg/ml | $357.00 | |||
CYP1A1 Antibody (B-4) Alexa Fluor® 790 | sc-25304 AF790 | 200 µg/ml | $357.00 |