CAPON Antibody (C-9): sc-374504
- CAPON Antibody (C-9) is a mouse monoclonal IgG1 κ CAPON antibody provided at 200 µg/ml
- raised against amino acids 304-503 mapping at the C-terminus of CAPON of rat origin
- CAPON Antibody (C-9) is recommended for detection of CAPON of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-CAPON Antibody (C-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CAPON (C-9): sc-374504.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CAPON Antibody (C-9) for WB applications. These reagents are now offered in bundles with CAPON Antibody (C-9) (see ordering information below).
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SEE ALSO...
CAPON Antibody (C-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects CAPON protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CAPON antibody (C-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CAPON, or carboxy-terminal PDZ ligand of nNOS, plays a crucial role in the regulation of neuronal nitric oxide synthase (nNOS) by selectively binding to the 100 amino acid PDZ domain of nNOS, while not interacting with endothelial or inducible NOS. This interaction is vital as CAPON sequesters nNOS in the cytosol, thereby influencing the biosynthesis of nitric oxide (NO), a key neurotransmitter involved in various physiological processes. The proper functioning of nNOS depends on association with synaptic proteins such as syntrophin, postsynaptic density protein 95 (PSD95), and PSD93, which facilitate transport to the plasma membrane where nNOS becomes catalytically active through NMDA-receptor mediated calcium channels. CAPON regulates nNOS association with PSD95 and PSD93 by competing for binding to the PDZ domain, thus preventing translocation and activation of nNOS. This regulatory mechanism maintains the balance of nitric oxide signaling in the nervous system, highlighting CAPON′s importance in synaptic function and neurophysiology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CAPON Antibody (C-9) References:
- Interaction of neuronal nitric-oxide synthase with alpha1-syntrophin in rat brain. | Hashida-Okumura, A., et al. 1999. J Biol Chem. 274: 11736-41. PMID: 10206989
- A new type of PDZ domain recognition. | Oschkinat, H. 1999. Nat Struct Biol. 6: 408-10. PMID: 10331862
- Solution structure of the extended neuronal nitric oxide synthase PDZ domain complexed with an associated peptide. | Tochio, H., et al. 1999. Nat Struct Biol. 6: 417-21. PMID: 10331866
- Domain interaction between NMDA receptor subunits and the postsynaptic density protein PSD-95. | Kornau, HC., et al. 1995. Science. 269: 1737-40. PMID: 7569905
- PDZ domain of neuronal nitric oxide synthase recognizes novel C-terminal peptide sequences. | Stricker, NL., et al. 1997. Nat Biotechnol. 15: 336-42. PMID: 9094134
- CAPON: a protein associated with neuronal nitric oxide synthase that regulates its interactions with PSD95. | Jaffrey, SR., et al. 1998. Neuron. 20: 115-24. PMID: 9459447
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CAPON Antibody (C-9) | sc-374504 | 200 µg/ml | $316.00 | |||
CAPON Antibody (C-9): m-IgG Fc BP-HRP Bundle | sc-529758 | 200 µg Ab; 10 µg BP | $354.00 | |||
CAPON Antibody (C-9): m-IgGκ BP-HRP Bundle | sc-522760 | 200 µg Ab, 40 µg BP | $354.00 | |||
CAPON Antibody (C-9): m-IgG1 BP-HRP Bundle | sc-543674 | 200 µg Ab; 20 µg BP | $354.00 | |||
CAPON Antibody (C-9) AC | sc-374504 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CAPON Antibody (C-9) HRP | sc-374504 HRP | 200 µg/ml | $316.00 | |||
CAPON Antibody (C-9) FITC | sc-374504 FITC | 200 µg/ml | $330.00 | |||
CAPON Antibody (C-9) PE | sc-374504 PE | 200 µg/ml | $343.00 | |||
CAPON Antibody (C-9) Alexa Fluor® 488 | sc-374504 AF488 | 200 µg/ml | $357.00 | |||
CAPON Antibody (C-9) Alexa Fluor® 546 | sc-374504 AF546 | 200 µg/ml | $357.00 | |||
CAPON Antibody (C-9) Alexa Fluor® 594 | sc-374504 AF594 | 200 µg/ml | $357.00 | |||
CAPON Antibody (C-9) Alexa Fluor® 647 | sc-374504 AF647 | 200 µg/ml | $357.00 | |||
CAPON Antibody (C-9) Alexa Fluor® 680 | sc-374504 AF680 | 200 µg/ml | $357.00 | |||
CAPON Antibody (C-9) Alexa Fluor® 790 | sc-374504 AF790 | 200 µg/ml | $357.00 |