ULK1 Antibody (F-4): sc-390904

ULK1 Antibody (F-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects ULK1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-ULK1 antibody (F-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ULK1 plays a crucial role in initiating autophagy, a vital cellular process that degrades and recycles cellular components, maintaining cellular homeostasis and responding to stress conditions. This function is particularly important in various diseases, including cancer and neurodegenerative disorders, where autophagy can influence cell survival and death. ULK1 forms a complex with ULK2, and both proteins share significant homology with UNC-51 kinase from Caenorhabditis elegans and APG1 kinase in yeast, which are also involved in autophagy regulation. Mapping to human chromosome 12q24.3, ULK1 is ubiquitously expressed across tissues, highlighting its fundamental role in cellular processes. ULK1′s ability to auto-phosphorylate its proline/serine-rich domain further underscores its regulatory capabilities, making ULK1 (F-4) monoclonal antibody an essential tool for researchers studying autophagy and its implications in health and disease.

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ULK1 Antibody (F-4) References:

1. Mouse ULK2, a novel member of the UNC-51-like protein kinases: unique features of functional domains.  |  Yan, J., et al. 1999. Oncogene. 18: 5850-9. PMID: 10557072
2. A mouse serine/threonine kinase homologous to C. elegans UNC51 functions in parallel fiber formation of cerebellar granule neurons.  |  Tomoda, T., et al. 1999. Neuron. 24: 833-46. PMID: 10624947
3. AMPK and mTOR regulate autophagy through direct phosphorylation of Ulk1.  |  Kim, J., et al. 2011. Nat Cell Biol. 13: 132-41. PMID: 21258367
4. SQSTM1/p62 activates NFE2L2/NRF2 via ULK1-mediated autophagic KEAP1 degradation and protects mouse liver from lipotoxicity.  |  Lee, DH., et al. 2020. Autophagy. 16: 1949-1973. PMID: 31913745
5. MAPK1/3 kinase-dependent ULK1 degradation attenuates mitophagy and promotes breast cancer bone metastasis.  |  Deng, R., et al. 2021. Autophagy. 17: 3011-3029. PMID: 33213267
6. Empagliflozin attenuates cardiac microvascular ischemia/reperfusion through activating the AMPKα1/ULK1/FUNDC1/mitophagy pathway.  |  Cai, C., et al. 2022. Redox Biol. 52: 102288. PMID: 35325804
7. Metrnl ameliorates diabetic cardiomyopathy via inactivation of cGAS/STING signaling dependent on LKB1/AMPK/ULK1-mediated autophagy.  |  Lu, QB., et al. 2022. J Adv Res.. PMID: 36334887
8. ULK1-mediated metabolic reprogramming regulates Vps34 lipid kinase activity by its lactylation.  |  Jia, M., et al. 2023. Sci Adv. 9: eadg4993. PMID: 37267363
9. Caenorhabditis elegans unc-51 gene required for axonal elongation encodes a novel serine/threonine kinase.  |  Ogura, K., et al. 1994. Genes Dev. 8: 2389-400. PMID: 7958904
10. Apg1p, a novel protein kinase required for the autophagic process in Saccharomyces cerevisiae.  |  Matsuura, A., et al. 1997. Gene. 192: 245-50. PMID: 9224897
11. Identification of mouse ULK1, a novel protein kinase structurally related to C. elegans UNC-51.  |  Yan, J., et al. 1998. Biochem Biophys Res Commun. 246: 222-7. PMID: 9600096
12. Human ULK1, a novel serine/threonine kinase related to UNC-51 kinase of Caenorhabditis elegans: cDNA cloning, expression, and chromosomal assignment.  |  Kuroyanagi, H., et al. 1998. Genomics. 51: 76-85. PMID: 9693035

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ULK1 Antibody (F-4) has 42 citations in a variety of scientific publications.

Primary antibodies like Anti-ULK1 Antibody (F-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ULK1 Antibody (F-4) Specifications

Alternate Product Name: ULK1 kinase

Product Clone Name: F-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 511-750

Epitope Target: ULK1

Epitope Species: human

Product Reactivity/Detection: ULK1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 42 citations in various scientific publications

Available Conjugates/Forms for ULK1 Antibody (F-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin