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PKC alpha Antibody (H-7) - Western Blotting - Image 387579
Simultaneous direct near-infrared western blot analysis of PKC α expression, detected with PKC α Antibody (H-7) Alexa Fluor® 790: sc-8393 AF790 and GAPDH expression, detected with GAPDH (G-9) Alexa Fluor® 680: sc-365062 AF680 in MOLT-4 (A), Jurkat (B), HeLa (C) and NIH/3T3 (D) whole cell lysates. Blocked with UltraCruz® Blocking Reagent: sc-516214.
Simultaneous direct near-infrared western blot analysis of PKC α expression, detected with PKC α Antibody (H-7) Alexa Fluor® 790: sc-8393 AF790 and GAPDH expression, detected with GAPDH (G-9) Alexa Fluor® 680: sc-365062 AF680 in MOLT-4 (A), Jurkat (B), HeLa (C) and NIH/3T3 (D) whole cell lysates. Blocked with UltraCruz® Blocking Reagent: sc-516214.

PKC alpha Antibody (H-7): sc-8393

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Datasheets
  • PKC alpha Antibody (H-7) is a mouse monoclonal IgG1 κ PKC alpha antibody, cited in 318 publications, provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 651-672 at the C-terminus of PKC α of human origin
  • PKC alpha Antibody (H-7) is recommended for detection of PKC α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • Anti-PKC alpha Antibody (H-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PKC alpha (H-7): sc-8393.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PKC alpha Antibody (H-7) for WB and IHC(P) applications. These reagents are now offered in bundles with PKC alpha Antibody (H-7) (see ordering information below).

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Additional PKC Antibodies including PKC β, PKC δ, PKC ε, PKC η, PKC γ, PKC ι, PKC λ, PKC μ, PKC ν and PKC θ

PKC α Antibody (H-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects PKC alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PKC α Antibody (H-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PKC alpha protein, a member of the protein kinase C (PKC) family, plays a crucial role in regulating numerous cellular functions such as cell growth, differentiation, gene expression, and hormone secretion, making PKC alpha vital for maintaining cellular homeostasis. PKC alpha is primarily located in the cytoplasm and translocates to the cell membrane upon activation, which is essential for PKC alpha′s role in signal transduction pathways. This translocation is triggered by diacylglycerols (DAG) and tumor-promoting phorbol esters, which bind to and activate PKC alpha, allowing PKC alpha to phosphorylate target proteins and modulate various cellular responses. The diverse expression patterns of PKC isoforms across different tissues highlight PKC alpha′s importance in specific signaling pathways, and PKC alpha′s unique cofactor dependencies further underscore PKC alpha′s distinct regulatory mechanisms within the PKC family.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

PKC alpha Antibody (H-7) References:

  1. The role of protein kinase C-alpha (PKC-alpha) in melanoma.  |  Lahn, MM. and Sundell, KL. 2004. Melanoma Res. 14: 85-9. PMID: 15057036
  2. A new member of the protein kinase C family, nPKC theta, predominantly expressed in skeletal muscle.  |  Osada, S., et al. 1992. Mol Cell Biol. 12: 3930-8. PMID: 1508194
  3. Resveratrol regulates cellular PKC alpha and delta to inhibit growth and induce apoptosis in gastric cancer cells.  |  Atten, MJ., et al. 2005. Invest New Drugs. 23: 111-9. PMID: 15744586
  4. Structural and functional diversities of a family of signal transducing protein kinases, protein kinase C family; two distinct classes of PKC, conventional cPKC and novel nPKC.  |  Ohno, S., et al. 1991. Adv Enzyme Regul. 31: 287-303. PMID: 1877391
  5. Expression and characterization of protein kinase C-delta.  |  Olivier, AR. and Parker, PJ. 1991. Eur J Biochem. 200: 805-10. PMID: 1915352
  6. PKC-alpha modulates TGF-beta signaling and impairs podocyte survival.  |  Tossidou, I., et al. 2009. Cell Physiol Biochem. 24: 627-34. PMID: 19910703
  7. Coronavirus disease 2019 (COVID-19), human erythrocytes and the PKC-alpha/-beta inhibitor chelerythrine -possible therapeutic implication.  |  Ghashghaeinia, M., et al. 2020. Cell Cycle. 19: 3399-3405. PMID: 33305655
  8. Calcium-dependent activation of a multifunctional protein kinase by membrane phospholipids.  |  Takai, Y., et al. 1979. J Biol Chem. 254: 3692-5. PMID: 438153
  9. Turnover of inositol phospholipids and signal transduction.  |  Nishizuka, Y. 1984. Science. 225: 1365-70. PMID: 6147898
  10. The role of protein kinase C in cell surface signal transduction and tumour promotion.  |  Nishizuka, Y. Nature. 308: 693-8. PMID: 6232463
  11. Protein kinase C as a possible receptor protein of tumor-promoting phorbol esters.  |  Kikkawa, U., et al. 1983. J Biol Chem. 258: 11442-5. PMID: 6311812
  12. Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters.  |  Castagna, M., et al. 1982. J Biol Chem. 257: 7847-51. PMID: 7085651

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

PKC alpha Antibody (H-7)

sc-8393
200 µg/ml
$316.00

PKC alpha Antibody (H-7): m-IgG Fc BP-HRP Bundle

sc-528219
200 µg Ab; 10 µg BP
$354.00

PKC alpha Antibody (H-7): m-IgGκ BP-HRP Bundle

sc-520544
200 µg Ab, 40 µg BP
$354.00

PKC alpha Antibody (H-7): m-IgG1 BP-HRP Bundle

sc-542822
200 µg Ab; 20 µg BP
$354.00

PKC alpha Antibody (H-7) AC

sc-8393 AC
500 µg/ml, 25% agarose
$416.00

PKC alpha Antibody (H-7) HRP

sc-8393 HRP
200 µg/ml
$316.00

PKC alpha Antibody (H-7) FITC

sc-8393 FITC
200 µg/ml
$330.00

PKC alpha Antibody (H-7) PE

sc-8393 PE
200 µg/ml
$343.00

PKC alpha Antibody (H-7) Alexa Fluor® 488

sc-8393 AF488
200 µg/ml
$357.00

PKC alpha Antibody (H-7) Alexa Fluor® 546

sc-8393 AF546
200 µg/ml
$357.00

PKC alpha Antibody (H-7) Alexa Fluor® 594

sc-8393 AF594
200 µg/ml
$357.00

PKC alpha Antibody (H-7) Alexa Fluor® 647

sc-8393 AF647
200 µg/ml
$357.00

PKC alpha Antibody (H-7) Alexa Fluor® 680

sc-8393 AF680
200 µg/ml
$357.00

PKC alpha Antibody (H-7) Alexa Fluor® 790

sc-8393 AF790
200 µg/ml
$357.00

PKC alpha (H-7) Neutralizing Peptide

sc-8393 P
100 µg/0.5 ml
$68.00

does this product work on zebrafish embryo?any suggestion for zebrafish use,the product SC-216 is not available now!

Asked by: liyana
Thank you for your question. We are not aware of any publications citing use of this mouse monoclonal antibody, PKC α (H-7): sc-8393, in zebrafish samples. The antibody is raised against a peptide that does share significant (76%) sequence identity with PKC α in zebrafish, so there is a good chance that it would work in this species. For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to request a sample to test in zebrafish.
Answered by: Technical Support
Date published: 2017-07-18

What secondary detection reagent should be used with PKC α Antibody (H-7) for Western blot?

Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 8
Date published: 2021-10-29
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Rated 5 out of 5 by from well function in WB and IFGood results when tested PKC alpha in western blot of whole cell extracts or IFfrom rat articular chondrocytes.
Date published: 2023-12-22
Rated 5 out of 5 by from Dendritic morphology of bipolar cells in wild type10 to 15 weeks old mice were anesthetized with isoflurane (Vetflurane®k, Virbac) and killed by cervical dislocation. Vertical sections: The following steps were conducted at room temperature if not stated otherwise. Eyes were quickly removed from the eye socket, opened at the scleral-corneal rim and immersed for 10 min with 4% paraformaldehyde (PFA) in 1X phosphate-buffered saline (1X PBS, pH 7.4). Cornea, lens and vitreous were removed. Eye cups were then fixed with 4%PFA/1X PBS for 20 min, washed four times with 1X PBS and cryoprotected by a graded sucrose series: 10% sucrose in 1X PBS for 1 h, 20% sucrose in 1X PBS for 1 h and 30% sucrose in 1X PBS overnight at 4 °C. Eyecups were orientated along the dorsoven-tral axis, embedded in OCT Medium (Tissue-Tek O.C.T Compound; Sakura Finetek, Tokyo, Japan) and frozen in liquid nitrogen. Vertical sections (16 μm) were cut on a cryostat (Leica Microsystems, Wetzlar, Germany), mounted on gelatine coated slides and stored at − 20 °C. For immunofluorescence experiments, sections were washed three times in 1X PBS-T (1X PBS + 0.1% Triton X-100, Sigma-Aldrich, St. Louis, MO, USA), blocked for 1 h in 1X PBS-T containing 1% bovine serum albumin (BSA, Sigma-Aldrich, A7030) and incubated overnight at 4 °C with primary antibodies diluted in 1X PBS-T at concentrations listed in Supplementary table 1. After wash-ing three times with 1X PBS-T, sections were incubated with the secondary antibodies (Supplementary table 2) for one. Additional washes preceded the counterstaining with DAPI (1:10,000; Sigma, D-9542) and eventually the sections were mounted using Poly/Mount (Polysciences, Inc., Warrington, PA, USA)
Date published: 2022-01-17
Rated 5 out of 5 by from Works great for IHC in mouse retinaWe use this antibody as standard marker in our lab. Very intense and nice staining of bipolar cells in the retina
Date published: 2022-01-05
Rated 5 out of 5 by from Works very good!Used this antibody for IF (1:50/1:100/1:200) on cryosections (10µm) from human retinal organoids and obtained nice images (for all dilutions)!
Date published: 2019-07-23
Rated 5 out of 5 by from Works great on pig retinaPKCa staining (IHC-Fr) on 8um thick pig retina for bipolar cells Blocking: 5% donkey serum P Ab: 1:50 (overnight) S Ab: anti-mouse 1:200 (1h)
Date published: 2017-10-23
Rated 5 out of 5 by from Nice AntibodyPKC α Antibody gives very good signal and manipulating conveniently with good control effect.The clarity of result can be  too good for us.
Date published: 2017-10-18
Rated 5 out of 5 by from Great productWe used this antibody for our western blot. give us good results.
Date published: 2017-09-27
Rated 5 out of 5 by from IHCThe relationship between the expression of PKC- alpha protein and the clinicopathological features of lung cancer was detected by IHC with this antibody.It is found that PKC-A is closely related to the development of lung cancer. We found PKA-A canbe used assit for lung cancer diagnosis.We recommend the use of 1:300 for immunohistochemistry.
Date published: 2017-09-16
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PKC alpha Antibody (H-7) is rated 4.8 out of 5 by 34.
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