Akt2 Antibody (F-7): sc-5270
- Akt2 Antibody (F-7) is a mouse monoclonal IgG1 κ, cited in 56 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 454-470 at the C-terminus of Akt2 of human origin
- Anti-Akt2 Antibody (F-7) is recommended for detection of Akt2 of mouse, rat and human origin by WB, IP, IF and IHC(P)
- Anti-Akt2 Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Akt2 (F-7): sc-5270.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Akt2 Antibody (F-7). This work is in progress.
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Akt2 Antibody (F-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Akt2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Akt2 Antibody (F-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Akt2, a member of the serine/threonine kinase Akt family, plays a crucial role in cellular signaling pathways that regulate metabolism, cell growth, and survival. Specifically, Akt2 is involved in the insulin signaling pathway, where activation occurs by phosphatidylinositol 3-kinase (PI3K) in response to insulin and insulin-like growth factor 1 (IGF-1). This activation is essential for promoting glucose uptake and glycogen synthesis, making Akt2 a key player in maintaining glucose homeostasis and energy balance in the body. Akt2′s ability to modulate various cellular processes, including apoptosis and cellular proliferation, underscores its importance in both normal physiology and disease states, such as cancer and metabolic disorders. A Pleckstrin homology domain in Akt2 allows interaction with phosphoinositides, facilitating recruitment to the plasma membrane and subsequent activation, which is vital for function in signaling cascades.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Akt2 Antibody (F-7) References:
- Identification of a human Akt3 (protein kinase B gamma) which contains the regulatory serine phosphorylation site. | Nakatani, K., et al. 1999. Biochem Biophys Res Commun. 257: 906-10. PMID: 10208883
- Mapping of AKT3, encoding a member of the Akt/protein kinase B family, to human and rodent chromosomes by fluorescence in situ hybridization. | Murthy, SS., et al. 2000. Cytogenet Cell Genet. 88: 38-40. PMID: 10773662
- Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction. | Burgering, BM. and Coffer, PJ. 1995. Nature. 376: 599-602. PMID: 7637810
- The protein kinase encoded by the Akt proto-oncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase. | Franke, TF., et al. 1995. Cell. 81: 727-36. PMID: 7774014
- AH/PH domain-mediated interaction between Akt molecules and its potential role in Akt regulation. | Datta, K., et al. 1995. Mol Cell Biol. 15: 2304-10. PMID: 7891724
- Amplification of AKT2 in human pancreatic cells and inhibition of AKT2 expression and tumorigenicity by antisense RNA. | Cheng, JQ., et al. 1996. Proc Natl Acad Sci U S A. 93: 3636-41. PMID: 8622988
- Protein kinase C modulates the insulin-stimulated increase in Akt1 and Akt3 activity in 3T3-L1 adipocytes. | Barthel, A., et al. 1998. Biochem Biophys Res Commun. 243: 509-13. PMID: 9480839
- Akt kinases and 2-deoxyglucose uptake in rat skeletal muscles in vivo: study with insulin and exercise. | Turinsky, J. and Damrau-Abney, A. 1999. Am J Physiol. 276: R277-82. PMID: 9887206
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Akt2 Antibody (F-7) | sc-5270 | 200 µg/ml | $316.00 | |||
Akt2 Antibody (F-7) AC | sc-5270 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Akt2 Antibody (F-7) HRP | sc-5270 HRP | 200 µg/ml | $316.00 | |||
Akt2 Antibody (F-7) FITC | sc-5270 FITC | 200 µg/ml | $330.00 | |||
Akt2 Antibody (F-7) PE | sc-5270 PE | 200 µg/ml | $343.00 | |||
Akt2 Antibody (F-7) Alexa Fluor® 488 | sc-5270 AF488 | 200 µg/ml | $357.00 | |||
Akt2 Antibody (F-7) Alexa Fluor® 546 | sc-5270 AF546 | 200 µg/ml | $357.00 | |||
Akt2 Antibody (F-7) Alexa Fluor® 594 | sc-5270 AF594 | 200 µg/ml | $357.00 | |||
Akt2 Antibody (F-7) Alexa Fluor® 647 | sc-5270 AF647 | 200 µg/ml | $357.00 | |||
Akt2 Antibody (F-7) Alexa Fluor® 680 | sc-5270 AF680 | 200 µg/ml | $357.00 | |||
Akt2 Antibody (F-7) Alexa Fluor® 790 | sc-5270 AF790 | 200 µg/ml | $357.00 | |||
Akt2 (F-7) Neutralizing Peptide | sc-5270 P | 100 µg/0.5 ml | $68.00 |