Smad3 Antibody (38-Q): sc-101154

Smad3 Antibody (38-Q) is a mouse monoclonal IgG2a kappa light chain antibody, specifically designed to detect Smad3 proteins from mouse, rat, and human origins across a variety of applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Smad3 antibody (38-Q) is raised against recombinant human Smad3. Smad3, also referred to as LDS3 and MADH3, is a critical intracellular mediator in the Transforming Growth Factor-beta (TGF-β) signaling pathway, playing a pivotal role in regulating gene expression related to cell proliferation, differentiation, apoptosis, and extracellular matrix production. By transducing signals from the cell membrane to the nucleus, Smad3 facilitates the transcription of target genes that are essential for maintaining cellular homeostasis and tissue development. Dysregulation of Smad3 activity is implicated in various pathological conditions, including fibrosis, cancer progression, and immune response modulation, making Smad3 a significant target for research and therapeutic intervention. Smad3 monoclonal antibody (38-Q) accurately detects and quantifies Smad3 protein levels, enabling researchers to explore the intricate mechanisms of TGF-β signaling and its impact on disease processes, thereby contributing to advancements in biomedical research and the development of novel treatments.

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Smad3 Antibody (38-Q) References:

1. The complexity of the Rab and Rho GTP-binding protein subfamilies revealed by a PCR cloning approach.  |  Chavrier, P., et al. 1992. Gene. 112: 261-4. PMID: 1555775
2. Global analysis of Smad2/3-dependent TGF-beta signaling in living mice reveals prominent tissue-specific responses to injury.  |  Lin, AH., et al. 2005. J Immunol. 175: 547-54. PMID: 15972691
3. Smads and chromatin modulation.  |  van Grunsven, LA., et al. 2005. Cytokine Growth Factor Rev. 16: 495-512. PMID: 15979924
4. TGF-beta signalling through the Smad pathway.  |  Massaous, J. and Hata, A. 1997. Trends Cell Biol. 7: 187-92. PMID: 17708943
5. Smad3 plays an inhibitory role in phosphate-induced vascular smooth muscle cell calcification.  |  Shimokado, A., et al. 2014. Exp Mol Pathol. 97: 458-64. PMID: 25303897
6. Smad3 promotes adverse cardiovascular remodeling and dysfunction in doxorubicin-treated hearts.  |  Cobb, MS., et al. 2022. Am J Physiol Heart Circ Physiol. 323: H1091-H1107. PMID: 36269647
7. MADR2 maps to 18q21 and encodes a TGFbeta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma.  |  Eppert, K., et al. 1996. Cell. 86: 543-52. PMID: 8752209
8. Receptor-associated Mad homologues synergize as effectors of the TGF-beta response.  |  Zhang, Y., et al. 1996. Nature. 383: 168-72. PMID: 8774881
9. Partnership between DPC4 and SMAD proteins in TGF-beta signalling pathways.  |  Lagna, G., et al. 1996. Nature. 383: 832-6. PMID: 8893010
10. Smad6 inhibits signalling by the TGF-beta superfamily.  |  Imamura, T., et al. 1997. Nature. 389: 622-6. PMID: 9335505
11. Smad8 mediates the signaling of the ALK-2 [corrected] receptor serine kinase.  |  Chen, Y., et al. 1997. Proc Natl Acad Sci U S A. 94: 12938-43. PMID: 9371779
12. TGF-beta signalling from cell membrane to nucleus through SMAD proteins.  |  Heldin, CH., et al. 1997. Nature. 390: 465-71. PMID: 9393997

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Smad3 Antibody (38-Q) has 128 citations in a variety of scientific publications.

Primary antibodies like Anti-Smad3 Antibody (38-Q) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Smad3 Antibody (38-Q) Specifications

Alternate Product Name: Smad3

Product Clone Name: 38-Q

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 120-222

Epitope Target: Smad3

Epitope Species: human

Product Reactivity/Detection: Smad3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 50 µg IgG_2a kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 128 citations in various scientific publications

Available Conjugates/Forms for Smad3 Antibody (38-Q): N/A