Smad2/3 Antibody (E-1): sc-376928
- Smad2/3 Antibody (E-1) is a mouse monoclonal IgG1 κ, cited in 6 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-29 at the N-terminus of Smad2 of human origin
- recommended for detection of Smad2 and Smad3 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- TransCruz reagent for ChIP application (sc-376928 X, 200 µg/0.1 ml)
- See Smad2/3 (C-8): sc-133098 for Smad2/3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Smad2/3 Antibody (E-1) for WB and IHC(P) applications. These reagents are now offered in bundles with Smad2/3 Antibody (E-1) (see ordering information below).
Smad2/3 Antibody (E-1) is a mouse monoclonal IgG1 kappa light chain antibody engineered to detect both Smad2 and Smad3 proteins across a wide range of species, including mouse, rat, human, equine, canine, bovine, porcine, and avian. anti-Smad2/3 antibody (E-1) is designed to recognize an epitope mapping to amino acids 2-29 at the N-terminus of human Smad2, ensuring precise identification of these pivotal signaling molecules. Smad2 and Smad3, also known as Mad2 and Mad3 respectively, are essential intracellular mediators of the Transforming Growth Factor-beta (TGFβ) signaling pathway. These proteins undergo critical post-translational modifications, such as phosphorylation at their C-terminal SSXS motifs, which are vital for their activation, dimerization with Smad4, and subsequent translocation to the nucleus where they regulate the transcription of target genes involved in processes like cell proliferation, differentiation, and apoptosis. Additionally, Smad2 and Smad3 can be ubiquitinated and sumoylated, modifications that play key roles in regulating their stability and transcriptional activity. The structural integrity and functional dynamics of Smad2/3 are fundamental for maintaining cellular homeostasis and responding to extracellular signals. Smad2/3 monoclonal antibody (E-1) is versatile in its applications, performing effectively in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). By facilitating detailed studies of Smad2 and Smad3-mediated signaling pathways and their post-translational modifications, Smad2/3 (E-1) antibody supports research into various physiological and pathological conditions, including cancer, fibrosis, and developmental disorders. Available in both non-conjugated and multiple conjugated forms, including horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and Alexa Fluor® variants, Smad2/3 (E-1) monoclonal antibody is an indispensable tool for researchers exploring the complexities of cellular signaling and gene regulation.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Smad2/3 Antibody (E-1) | sc-376928 | 200 µg/ml | $316.00 | |||
Smad2/3 Antibody (E-1): m-IgG Fc BP-HRP Bundle | sc-538013 | 200 µg Ab; 10 µg BP | $354.00 | |||
Smad2/3 Antibody (E-1): m-IgGκ BP-HRP Bundle | sc-535530 | 200 µg Ab; 40 µg BP | $354.00 | |||
Smad2/3 Antibody (E-1): m-IgG1 BP-HRP Bundle | sc-545485 | 200 µg Ab; 20 µg BP | $354.00 | |||
Smad2/3 (E-1) Neutralizing Peptide | sc-376928 P | 100 µg/0.5 ml | $68.00 | |||
Smad2/3 Antibody (E-1) X | sc-376928 X | 200 µg/0.1 ml | $316.00 |