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ITF-2/TCF4 Antibody (367.2): sc-101095

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Datasheets
  • ITF-2/TCF4 Antibody (367.2) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 100 µg/ml
  • raised against recombinant ITF-2 of human origin
  • recommended for detection of ITF-2 of mouse, rat and human origin by WB, IP and ELISA
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ITF-2/TCF4 Antibody (367.2). This work is in progress.

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ITF-2 Antibody (367.2) is a mouse monoclonal IgG1 antibody that detects immunoglobulin transcription factor 2 (ITF-2) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). ITF-2 plays a crucial role in the regulation of gene expression, particularly in muscle development, by binding to specific DNA motifs such as the immunoglobulin enhancer Mu-E5/KE5-motif and the Ephrussi-box (E-box) element found in SSTR2-INR. Anti-ITF-2 antibody (367.2) recognizes a transcription factor essential for activating muscle-specific genes, with the ability to form homo- or hetero-oligomers with other myogenic factors like myogenin and MyoD. ITF-2 expression in various tissues, including the adult heart, brain, placenta, and skeletal muscle, highlights a ubiquitous role in cellular processes. Additionally, alternatively spliced isoforms of ITF-2 allow for nuanced regulation of transcription, enabling either activation or repression of target genes, which is vital for maintaining proper muscle function and development. ITF-2 monoclonal antibody (367.2) serves as an invaluable tool for researchers studying muscle biology and transcriptional regulation.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

ITF-2/TCF4 Antibody (367.2)

sc-101095
100 µg/ml
$333.00

I am looking for anti-ITF-2 (N-16; Santa Cruz) or anti-ITF-2 (K-12; Santa Cruz) primary antibodies but on searching these N-16 and K-12 antibodies I am getting ITF2 (367.2) is it same

Asked by: shariq
Thank you for your question. The two antibodies you are searching for are polyclonal antibodies that are no longer available. We currently offer mouse monoclonal antibodies against ITF-2. Please contact our Technical Service Department for help in choosing the best antibody for your research.
Answered by: Technical Support
Date published: 2020-06-03

For Western Blot, is it recommended to use denatured or non-denatured conditions with ITF-2 (367.2): sc-101095 antibody?

Asked by: TinTin
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 1 out of 5 by from Antibody can NOT distinguish between FL & TR TCF4Unlike SantaCruz's goat anti-ITf2 (N16) [discontinued], this monoclonal antibody failed to distinguish between truncated and full length TCF4 protein lysates from mouse frontal cortex tissue. It produced what seems to be a false band in the tr/tr lane at the same size as the fl/fl. The kDa difference should be around 7-10kda, but nothing is seen. Personally, I wouldn't recommend this product for TCF4 expression.
Date published: 2018-07-24
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ITF-2/TCF4 Antibody (367.2) is rated 2.0 out of 5 by 2.
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