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p-ATF-2 Antibody (103C411): sc-52941

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Datasheets
  • p-ATF-2 Antibody (103C411) is a mouse monoclonal IgG1, cited in 2 publications, provided at 100 µg/ml
  • raised against a peptide containing phosphorylated Thr-71 of ATF-2 of human origin
  • recommended for detection of Thr 71 phosphorylated ATF-2 of human origin by WB
  • See p-ATF-2 (F-1): sc-8398 for p-ATF-2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-ATF-2 Antibody (103C411). This work is in progress.

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SEE ALSO...

p-ATF-2 Antibody (103C411) is a mouse monoclonal IgG1 antibody that detects p-ATF-2 in human samples through western blotting (WB). p-ATF-2, a phosphorylated form of ATF-2, plays a crucial role in regulating gene transcription, particularly in response to stress signals. p-ATF-2 is primarily located in the nucleus, where p-ATF-2 functions as a transcription factor binding to specific DNA sequences to modulate target gene expression. Nuclear localization of p-ATF-2 enables interaction with other transcription factors and co-regulators, influencing cellular processes like survival, differentiation, and apoptosis. p-ATF-2 regulates genes managing oxidative stress and inflammation, making p-ATF-2 vital for cellular homeostasis. Located on chromosome 2q31.1, human p-ATF-2 is critical for transcriptional response to environmental stimuli, highlighting p-ATF-2′s importance in normal physiology and disease states.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

p-ATF-2 Antibody (103C411)

sc-52941
100 µg/ml
$316.00

How much sample do you recommend loading on the gel for Western blot detection using p-ATF2 (103C411): sc-52941?

Asked by: Randy McDonald
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-27
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Rated 4 out of 5 by from Published WB results using 1:500 dilutionPublished WB results using 1:500 dilution using LNCaP, C4, C4-2, and C4-2B cell lines. -SCBT Publication Review
Date published: 2013-02-26
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p-ATF-2 Antibody (103C411) is rated 4.0 out of 5 by 1.
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