Aprataxin Antibody (B-12): sc-374108
- Aprataxin Antibody (B-12) is a mouse monoclonal IgG2a κ Aprataxin antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 57-356 mapping at the C-terminus of Aprataxin of human origin
- Aprataxin Antibody (B-12) is recommended for detection of Aprataxin isoforms 1-10 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Aprataxin Antibody (B-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-374108 X, 200 µg/0.1 ml)
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Aprataxin (B-12): sc-374108.
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Aprataxin Antibody (B-12) for WB and IHC(P) applications. These reagents are now offered in bundles with Aprataxin Antibody (B-12) (see ordering information below).
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Aprataxin Antibody (B-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects Aprataxin of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Aprataxin antibody (B-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Aprataxin is primarily located in the nucleus, specifically within the nucleoplasm and nucleolus, where Aprataxin plays a crucial role in DNA single-strand break repair. This nuclear localization enables Aprataxin to effectively mediate protein-protein interactions with other molecules involved in cellular response to DNA damage, thereby maintaining genomic stability. Aprataxin belongs to the histidine triad (HIT) superfamily and features three conserved domains: an N-terminal forkhead-associated (FHA) domain that facilitates these interactions, a HIT domain similar to Hint, and a C-terminal zinc finger domain. Mutations in the APTX gene, which encodes for Aprataxin, can lead to protein destabilization and are associated with a rare neurological disorder known as ataxia-oculomotor apraxia, characterized by abnormal head and eye movements. These mutations often affect the HIT domain or result in truncation before the zinc finger domain, underscoring Aprataxin′s importance in cellular repair mechanisms and neurological health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Aprataxin Antibody (B-12) References:
- Aprataxin, a novel protein that protects against genotoxic stress. | Gueven, N., et al. 2004. Hum Mol Genet. 13: 1081-93. PMID: 15044383
- The novel human gene aprataxin is directly involved in DNA single-strand-break repair. | Mosesso, P., et al. 2005. Cell Mol Life Sci. 62: 485-91. PMID: 15719174
- Disease-associated mutations inactivate AMP-lysine hydrolase activity of Aprataxin. | Seidle, HF., et al. 2005. J Biol Chem. 280: 20927-31. PMID: 15790557
- [Mutation of the aprataxin gene presenting with Charcot-Marie-Tooth-like neuropathy and cerebellar ataxia]. | Ochsner, F., et al. 2005. Rev Neurol (Paris). 161: 331-6. PMID: 15800456
- Very late onset in ataxia oculomotor apraxia type I. | Criscuolo, C., et al. 2005. Ann Neurol. 57: 777. PMID: 15852392
- Ataxia-oculomotor apraxia syndrome. | Gascon, GG., et al. 1995. J Child Neurol. 10: 118-22. PMID: 7782601
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aprataxin Antibody (B-12) | sc-374108 | 200 µg/ml | $316.00 | |||
Aprataxin Antibody (B-12): m-IgG Fc BP-HRP Bundle | sc-529663 | 200 µg Ab; 10 µg BP | $354.00 | |||
Aprataxin Antibody (B-12): m-IgGκ BP-HRP Bundle | sc-522625 | 200 µg Ab, 40 µg BP | $354.00 | |||
Aprataxin Antibody (B-12): m-IgG2a BP-HRP Bundle | sc-547354 | 200 µg Ab; 10 µg BP | $354.00 | |||
Aprataxin Antibody (B-12) X | sc-374108 X | 200 µg/0.1 ml | $316.00 | |||
Aprataxin Antibody (B-12) AC | sc-374108 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Aprataxin Antibody (B-12) HRP | sc-374108 HRP | 200 µg/ml | $316.00 | |||
Aprataxin Antibody (B-12) FITC | sc-374108 FITC | 200 µg/ml | $330.00 | |||
Aprataxin Antibody (B-12) PE | sc-374108 PE | 200 µg/ml | $343.00 | |||
Aprataxin Antibody (B-12) Alexa Fluor® 488 | sc-374108 AF488 | 200 µg/ml | $357.00 | |||
Aprataxin Antibody (B-12) Alexa Fluor® 546 | sc-374108 AF546 | 200 µg/ml | $357.00 | |||
Aprataxin Antibody (B-12) Alexa Fluor® 594 | sc-374108 AF594 | 200 µg/ml | $357.00 | |||
Aprataxin Antibody (B-12) Alexa Fluor® 647 | sc-374108 AF647 | 200 µg/ml | $357.00 | |||
Aprataxin Antibody (B-12) Alexa Fluor® 680 | sc-374108 AF680 | 200 µg/ml | $357.00 | |||
Aprataxin Antibody (B-12) Alexa Fluor® 790 | sc-374108 AF790 | 200 µg/ml | $357.00 |