Recombinant Bovine TNF-alpha Protein

Name Recombinant Bovine TNF-alpha Protein
Supplier R&D Systems
Catalog 2279-BT
Category Protein
Prices $289.00
Sizes 25 µg
Species Reactivities Bovine
Nature Recombinant
Source E. coli -derived Leu78-Leu234, with an N-terminal Met
Purity >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Bioactivity Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al . (eds): IRL Press. 221. The ED 50 for this effect is 0.025-0.1 µg/mL. When cytotoxicity is measured on the porcine PK-15 cells, the ED 50 is 0.05-0.15 ng/mL.
Endotoxin <0.10 EU per 1 μg of the protein by the LAL method.
SwissProt/Accession Q06599
Gene TNF
Sequence Met
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