
Densitometry of protein isoforms visualised by 2-DE. The densitometry scan demonstrates the purified Human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.

1D SDS-PAGE of ab83824 before and after treatment with glycosidases to remove oligosaccharides. Lane 1: ab83824 Lane 2: ab83824 treated with PNGase F to remove potential N-linked glycans 10 μg protein loaded per lane; Deep Purple™ stained. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans.