Active human B Raf (mutated V600 E) protein fragment
| Name | Active human B Raf (mutated V600 E) protein fragment |
|---|---|
| Supplier | Abcam |
| Catalog | ab196061 |
| Category | Protein |
| Prices | $593.00 |
| Sizes | 10 µg |
| Applications | SDS-PAGE FA |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | Baculovirus infected sf9 cells |
| Tag/Conjugation | GST tag N-Terminus |
| Purity | >= 62 % by SDS-PAGE. |
| Bioactivity | Specific activity 150 pmol/min/μg. Assay was performed in a kinase buffer containing 0.1 mg/ml inactive MEK1 substrate and 10 μM ATP. Reaction was done at 30°C for 30 minutes. ATP transferred was calculated using Kinase reagents. |
| SwissProt/Accession | P15056 |
| Gene | BRAF |
| Residue | 417 to 766 |
| Sequence | MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDPMGHHHHHHGRRRASVAAGILVPRGSPGLD GIYARGIQQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQIT VGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKT RHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIAR QTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATEKSRWSGSH QFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSN INNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFP QILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAG GYGAFPVH |
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