| Name | Ubiquitin protein (Mutated K48) (His tag), Chemical Free |
|---|---|
| Supplier | Abcam |
| Catalog | ab80740 |
| Category | Protein |
| Applications | Conjugation SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | E. coli |
| Tag/Conjugation | His tag N-Terminus |
| Purity | > 95 % by SDS-PAGE. |
| Bioactivity | Mutation of lysine 48 to arginine renders ubiquitin (Ub) unable to form poly-Ub chains via lysine 48 linkages with other Ub molecules. ab80740 can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins (monoubiquitination). Ideal for the reduction in poly-Ub chain length/conjugation rates and for the determination of poly-Ub chains specificity. |
| SwissProt/Accession | P62988 |
| Gene | UBC |
| Supplier Page | Shop |