Recombinant TAF1 (1398-1524) protein gel. TAF1 (1398-1524) protein was run on an SDS-PAGE gel and stained with Coomassie blue.
Recombinant TAF1 (1398-1524) activity using AlphaScreen. TAF1 (1398-1524) activity was assessed using an AlphaScreen® assay testing various buffer formulations. This data was generated and kindly provided courtesy of ChemPartner.
HTRF Assay for Recombinant TAF1 (1398-1524) protein activity. 3 µM histone peptide H4K5/K8/K12/K16 (4ac) was incubated with TAF1 (1398-1524) protein in reaction buffer including 50 mM HEPES-NaOH pH 7. 0, 0. 1% BSA for 1 hour at room temperature. Anti-Flag antibody was used to detect reaction products.