Recombinant SP140 (688-862) protein

Name	Recombinant SP140 (688-862) protein
Supplier	ActiveMotif
Catalog	31448
Category	Protein
Prices	$395.00
Sizes	100 μg
Species Reactivities	Human
Nature	Recombinant
Source	E. coli
Gene	SP140
Description	The peptide corresponding to amino acids 688-862 that contains the bromodomain sequences of SP140 (accession number NP_009168.4) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 26.98 kDa
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Product images

Recombinant SP140 (688-862) protein gel. SP140 (688-862) protein was run on an SDS-PAGE gel and stained with Coomassie blue.

Recombinant SP140 (688-862) activity using AlphaScreen. SP140 (688-862) was used in an AlphaScreen® assay to generate a titration curve demonstrating the signal response in the presence of modified peptide substrate at increasing protein concentrations. This data was generated and kindly provided courtesy of ChemPartner.

HTRF Assay for Recombinant SP140 (688-862) protein activity. 3 µM histone peptide H3K14ac was incubated with SP140 (688-862) in reaction buffer including 50 mM HEPES-NaOH pH 7. 0, 0. 1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.