Recombinant PHIP (1302-1434) protein gel. PHIP (1302-1434) protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue.
HTRF assay for Recombinant PHIP (1302-1434) protein activity. 3 µM histone peptide H4K5/8/12/16 (ac4) was incubated with PHIP (1302-1434) in reaction buffer including 50 mM HEPES-NaOH pH 7. 0, 0. 1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.
HTRF assay for Recombinant PHIP (1302-1434) protein activity. 3 µM histone peptide H4K5/8/12/16 (ac4) was incubated with PHIP (1302-1434) in reaction buffer including 50 mM HEPES-NaOH pH 7. 0, 0. 1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.