Recombinant PCAF (715-829) protein
| Name | Recombinant PCAF (715-829) protein |
|---|---|
| Supplier | ActiveMotif |
| Catalog | 31370 |
| Category | Protein |
| Prices | $395.00, $3,500.00 |
| Sizes | 100 µg, 1 mg |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | E. coli |
| Gene | KAT2B |
| Description | The peptide corresponding to amino acids 715 - 829 that contains the bromodomain sequences of PCAF (accession number NM_003884.4) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 19.8 kDa |
| Supplier Page | Shop |
Product images
Recombinant PCAF (715-829) protein gel. PHIP (1302-1434) protein was run on an SDS-PAGE gel and stained with Coomassie blue.
Recombinant PCAF (715-829) activity using AlphaScreen. PCAF (715-829) activity was assessed using an AlphaScreen® assay testing various buffer formulations. This data was generated and kindly provided courtesy of ChemPartner.
HTRF Assay for Recombinant PCAF (715-829) protein activity. 3 µM histone peptide H4K5/K8/K12/K16 (4ac) was incubated with PCAF (715-829) protein in reaction buffer including 50 mM HEPES-NaOH pH 7. 0, 0. 1% BSA for 1 hour at room temperature, Anti-Flag antibody was used to detect reaction products.