
Exposure time : 10 minutes Lanes 1 to 10: ab12550 Rabbit polyclonal to PTPD1 (1/500) . Predicted band observed (~179kDa) in lanes 1-5 but not in lanes treated with ab12560 (blocking peptide). Lanes 1 to 10: 20µg of cell lysate per lane. Lanes 6 to 10: ab12550 + 1µg/ml blocking peptide (ab12560) Lane 1: HeLa Nuclear Extract Lane 2: HeLa Whole Cell Lysate Lane 3: A431 Whole Cell Lysate Lane 4: Jurkat Whole Cell Lysate Lane 5: HEK293 Whole Cell Lysate Lane 6: HeLa Nuclear Extract + blocking peptide (ab12560) Lane 7: HeLa Whole Cell Lysate + blocking peptide (ab12560) Lane 8: A431 Whole Cell Lysate + blocking peptide (ab12560) Lane 9: Jurkat Whole Cell Lysate + blocking peptide (ab12560) Lane 10: HEK293 Whole Cell Lysate + blocking peptide (ab12560)