Active human Protein C full length protein
| Name | Active human Protein C full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab198147 |
| Category | Protein |
| Prices | $858.00 |
| Sizes | 20 µg |
| Applications | SDS-PAGE FA |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | HEK 293 cells |
| Tag/Conjugation | His tag C-Terminus |
| Purity | >90% by SDS-PAGE. |
| Bioactivity | Specific Activity: 90 pmol/min/µg. Assay Conditions: 50 mM Tris-HCl, pH 8.5, 150 mM NaCl, 10 mM CaCl 2 , 0.01% Brij-35, 100 µM substrate, and various amounts of Activated Protein C. Incubate for 10 minutes at room temperature. Fluorescence intensity is measured at exc380/em460. |
| SwissProt/Accession | P04070 |
| Gene | PROC |
| Residue | 1 to 461 |
| Sequence | MWQLTSLLLF VATWGISGTP APLDSVFSSS ERAHQVLRIR KRANSFLEEL RHSSLERECI EEICDFEEAK EIFQNVDDTL AFWSKHVDGD QCLVLPLEHP CASLCCGHGT CIDGIGSFSC DCRSGWEGRF CQREVSFLNC SLDNGGCTHY CLEEVGWRRC SCAPGYKLGD DLLQCHPAVK FPCGRPWKRM EKKRSHLKRD TEDQEDQVDP RLIDGKMTRR GDSPWQVVLL DSKKKLACGA VLIHPSWVLT AAHCMDESKK LLVRLGEYDL RRWEKWELDL DIKEVFVHPN YSKSTTDNDI ALLHLAQPAT LSQTIVPICL PDSGLAEREL NQAGQETLVT GWGYHSSREK EAKRNRTFVL NFIKIPVVPH NECSEVMSNM VSENMLCAGI LGDRQDACEG DSGGPMVASF HGTWFLVGLV SWGEGCGLLH NYGVYTKVSR YLDWIHGHIR DKEAPQKSWA P |
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Product images
Specific activity of ab198147.
4-20% SDS-PAGE analysis of ab198147 (3 µg) with Coomassie staining. Note: Protein is secreted and converted to the active form with disulfide link (light chain a.a. 43-199, heavy chain a.a. 212-461). The signal peptide (a.a. 1-32), propeptide (a.a. 33-42), and activation peptide (a.a. 200-211) are cleaved. MW = ~60 kDa, ~42 kDa, ~20 kDa under reducing conditions.