Active human KMT1A / SUV39H1 protein fragment
| Name | Active human KMT1A / SUV39H1 protein fragment |
|---|---|
| Supplier | Abcam |
| Catalog | ab80289 |
| Category | Protein |
| Prices | $239.00 |
| Sizes | 25 µg |
| Applications | FA SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | E. coli |
| Tag/Conjugation | proprietary tag N-Terminus |
| Bioactivity | Specific Activity: 3.5 pmol/min/μg. Assay conditions: 50 μl reaction mix (50 mM TRIS pH 8.8, 5mM MgCl2, 4mM DTT, 0.05% Tween-20, 40 μM S-adenosylmethionine, and 10-100 ng SUV39H1) add to the wells coated with the substrate on a GBioscience Neutravidin black plate. Incubate for 1 hr. Add antibody against methylated K9 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence. |
| SwissProt/Accession | O43463 |
| Gene | SUV39H1 |
| Residue | 82 to 412 |
| Sequence | Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily. Contains 1 chromo domain. Contains 1 post-SET domain. Contains 1 pre-SET domain. Contains 1 SET domain. |
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