Lanes 1 to 8 (Histone 0.5 µ g/lane ( ab121 )) Lane 1: ab2886 (Histone H3 mono methyl K79) 1/500 Lane 2: ab2886 (Histone H3 mono methyl K79) 1/500 + ab4555 ( ab2886 ) (Histone H3 mono methyl K79) peptide 1 µ g/ml Lane 3: ab2886 (Histone H3 mono methyl K79) 1/500+ ab4556 ( ab3594 ) (Histone H3 Di methyl K79) peptide 1 µ g/ml Lane 4: ab2886 (Histone H3 mono methyl K79) 1/500+ ab2621 (Histone H3 tri methyl K79) peptide 1 µ g/ml Lane 5: ab2886 (Histone H3 mono methyl K79) 1/500+ ab4560 ( ab2885 ) (Histone H4 Di methyl K79) peptide 1 µ g/ml Lane 6: ab2886 (Histone H3 mono methyl K79) 1/500+ ab1771 (Histone H3 mono methyl K9) peptide 1 µ g/ml Lane 7: ab2886 (Histone H3 mono methyl K79) 1/500+ ab4264 ( ab4558 ) (Histone H3 non-modified) peptide 1 µ g/ml Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000) Exposure time: 5 minutes Expected mole
All lanes: (Histone 0.5µg/lane ( ab121 )) Lane 1 : ab2886 (Histone H3 mono methyl K79) 1/500 Lane 2 : ab2886 (Histone H3 mono methyl K79) 1/500 + ab4555 ( ab2886 ) (Histone H3 mono methyl K79) peptide 1µg/ml Lane 3 : ab2886 (Histone H3 mono methyl K79) 1/500+ ab4556 ( ab3594 ) (Histone H3 Di methyl K79) peptide 1µg/ml Lane 4 : ab2886 (Histone H3 mono methyl K79) 1/500+ ab2621 (Histone H3 tri methyl K79) peptide 1µg/ml Lane 5 : ab2886 (Histone H3 mono methyl K79) 1/500+ ab4560 ( ab2885 ) (Histone H4 Di methyl K79) peptide 1µg/ml Lane 6: ab2886 (Histone H3 mono methyl K79) 1/500+ ab1771 (Histone H3 mono methyl K9) peptide 1µg/ml Lane 7: ab2886 (Histone H3 mono methyl K79) 1/500+ ab4264 ( ab4558 ) (Histone H3 non-modified) peptide 1µg/ml Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000) Predicted band size : 15 kDa Observed band size : 14, 17 kDa Exposure time : 5 minutes
All lanes: (Histone 0.5µg/lane ( ab121 )) Lane 1 : ab2886 (Histone H3 mono methyl K79) 1/500 Lane 2 : ab2886 (Histone H3 mono methyl K79) 1/500 + ab4555 ( ab2886 ) (Histone H3 mono methyl K79) peptide 1µg/ml Lane 3 : ab2886 (Histone H3 mono methyl K79) 1/500+ ab4556 ( ab3594 ) (Histone H3 Di methyl K79) peptide 1µg/ml Lane 4 : ab2886 (Histone H3 mono methyl K79) 1/500+ ab2621 (Histone H3 tri methyl K79) peptide 1µg/ml Lane 5 : ab2886 (Histone H3 mono methyl K79) 1/500+ ab4560 ( ab2885 ) (Histone H4 Di methyl K79) peptide 1µg/ml Lane 6: ab2886 (Histone H3 mono methyl K79) 1/500+ ab1771 (Histone H3 mono methyl K9) peptide 1µg/ml Lane 7: ab2886 (Histone H3 mono methyl K79) 1/500+ ab4264 ( ab4558 ) (Histone H3 non-modified) peptide 1µg/ml Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000) Predicted band size : 15 kDa Observed band size : 14, 17 kDa Exposure time : 5 minutes