Active human IL12 full length protein
| Name | Active human IL12 full length protein |
|---|---|
| Supplier | Abcam |
| Catalog | ab89013 |
| Category | Protein |
| Prices | $389.00 |
| Sizes | 5 µg |
| Applications | FA SDS-PAGE |
| Species Reactivities | Human |
| Nature | Recombinant |
| Source | HEK 293 cells |
| Purity | > 95 % by SDS-PAGE. |
| Bioactivity | Activity was measured by its ability to stimulate the proliferation of PHA-activated human T-lymphoblasts, and is typically in the range of 0.05-0.20 ng/ml. |
| SwissProt/Accession | P29459 |
| Gene | IL12A |
| Sequence | Theoretical Sequence Alpha Chain: RNLPVATPDPGMFPCLHHSQNLLRAVSNMLQKARQTLEFYPC TSEEIDHE DITKDKTSTVEACLPLELTKNESCLNSRETSFITNGSCLA SRKTSFMMAL CLSSIYEDLKMYQVEFKTMNAKLLMDPKRQIFLDQNML AVIDELMQALNF NSETVPQKSSLEEPDFYKTKIKLCILLHAFRIRAVT IDRVMSYLNAS Beta Chain: IWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQ SSEVLGSG KTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIW STDILKDQKE PKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSR GSSDPQGVTCGA ATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPI EVMVDAVHKLKYEN YTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSW EYPDTWSTPHSYFSLT FCVQVQGKSKREKKDRVFTDKTSATVICRKNA SISVRAQDRYYSSSWSEW ASVPCS |
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Product images
1D SDS-PAGE of ab89013 before and after treatment with glycosidases to remove oligosaccharides. Lane 1: ab89013 Lane 2: ab89013 treated with PNGase F to remove potential N-linked glycans Lane 3: ab89013 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Appearance of additional band at lower MWt after treatment with PNGase F indicates the presence of N-linked glycans. The subsequent drop in MWt after treatment with a glycosidase cocktail indicates the presence of O-linked glycans. Additional high MWt bands in lane 3 are glycosidase enzymes.
A sample of ab89013 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 µg of protein was loaded. Spot trains indicate presence of multiple isoforms.
Densitometry of protein isoforms visualised by 2-DE. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification. The triangles indicate theoretical pI and MWt of the alpha and beta chains of the protein.