RFX1

Gene Symbol RFX1
Entrez Gene 5989
Alt Symbol EFC, RFX
Species Human
Gene Type protein-coding
Description regulatory factor X, 1 (influences HLA class II expression)
Other Description MHC class II regulatory factor RFX|MHC class II regulatory factor RFX1|enhancer factor C|regulatory factor X 1|trans-acting regulatory factor 1|transcription factor RFX1
Swissprots P22670
Accessions EAW84387 P22670 A20498 CAA01506 AI917511 AK309824 BC049826 AAH49826 BM557928 BM662273 X58964 CAA41730 XM_011528165 XP_011526467 XM_011528166 XP_011526468 XM_011528167 XP_011526469 XM_011528168 XP_011526470 XM_011528169 XP_011526471 XM_011528170 XP_011526472 NM_002918 NP_002909
Function Regulatory factor essential for MHC class II genes expression. Binds to the X boxes of MHC class II genes. Also binds to an inverted repeat (ENH1) required for hepatitis B virus genes expression and to the most upstream element (alpha) of the RPL30 promoter.
Subcellular Location Nucleus.

RFX1 (E-16) - sc-10651 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
RFX1 (E-16): sc-10651. Western blot analysis of RFX1 expression in HeLa (A) and K-562 (B) nuclear extracts.

RFX1 (F-6) - sc-374270 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
RFX1 (F-6): sc-374270. Western blot analysis of RFX1 expression in K-562 (A) and Jurkat (B) nuclear extracts.

RFX1 (H-11) - sc-166468 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
RFX1 (H-11): sc-166468. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

RFX1 (H-2) - sc-376041 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
RFX1 (H-2): sc-376041. Western blot analysis of RFX1 expression in K-562 (A) and Jurkat (B) whole cell lysates.

RFX1 (H-230) - sc-48809 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
RFX1 (H-230): sc-48809. Immuno?fluorescence staining of methanol-fixed HeLa cells showing nuclear local?ization.

RFX1 (I-19) - sc-10652 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
RFX1 (I-19): sc-10652. Immuno?fluorescence staining of methanol-fixed HeLa cells showing nuclear local?ization.

RFX1 (D-19) - sc-10650 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
RFX1 (D-19): sc-10650. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

Anti-RFX1 antibody - ab87171 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-RFX1 antibody (ab87171) at 1 µg/ml + Transfected 293T cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-RFX1 antibody - ab110927 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of RFX1 by Western Blot of Immunprecipitate. anti-RFX1 at 0.4µg/ml staining RFX1 in HeLa whole cell lysate immunoprecipitated using ab110927 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

Anti-RFX / RFX1 Antibody (aa896-925, AP) - LS-C268111 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFX / RFX1 Antibody (aa896-925, APC) - LS-C268112 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFX / RFX1 Antibody (aa896-925, Biotin) - LS-C268113 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFX / RFX1 Antibody (aa896-925, FITC) - LS-C268114 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFX / RFX1 Antibody (C-Terminus) - LS-C82041 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
MCF7 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.

Anti-RFX / RFX1 Antibody (aa896-925, Azide-free, HRP) - LS-C268115 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFX / RFX1 Antibody (aa38-87) - LS-C80186 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.

Anti-RFX / RFX1 Antibody (aa896-925, PE) - LS-C268116 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFX / RFX1 Antibody (aa287-336) - LS-C101681 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
RFX1 antibody LS-C101681 Western blot of Transfected 293T cell lysate.

Anti-RFX / RFX1 Antibody (aa181-230) - LS-C116025 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
RFX1 antibody LS-C116025 Western blot of Mouse Spleen lysate.

Anti-RFX / RFX1 Antibody (aa200-250) - LS-C289745 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RFX / RFX1 Antibody (aa929-979) - LS-C289748 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RFX / RFX1 Antibody (aa1-50) - LS-C289746 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RFX / RFX1 Antibody (aa895-925) - LS-C324628 from LifeSpan Bioscience

Prices $435.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFX / RFX1 Antibody (aa896-925) - LS-C162557 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Fluorescent image of U251 cell stained with RFX1 Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RFX1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). RFX1 immunoreactivity is localized to Nucleus significantly.

RFX1 Antibody - NBP1-52654 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: RFX1 Antibody [NBP1-52654] - Detection of Human RFX1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFX1 antibody NBP1-52654 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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