RAF1

Gene Symbol	RAF1
Entrez Gene	5894
Alt Symbol	CMD1NN, CRAF, NS5, Raf-1, c-Raf
Species	Human
Gene Type	protein-coding
Description	Raf-1 proto-oncogene, serine/threonine kinase
Other Description	C-Raf proto-oncogene, serine/threonine kinase|Oncogene RAF1|RAF proto-oncogene serine/threonine-protein kinase|proto-oncogene c-RAF|raf proto-oncogene serine/threonine protein kinase|v-raf-1 murine leukemia viral oncogene homolog 1
Swissprots	Q9UC20 B0LPH8 P04049 Q15278 B2R5N3
Accessions	AAA60247 AAP03432 ABY87557 AGC09606 CAD86886 CBX55729 CDL93489 EAW64134 EAW64135 P04049 AK226028 AK303561 BAG64584 AK303920 BAG64848 AK312248 BAG35180 BC018119 AAH18119 BQ221862 BU683389 BX648063 DC342671 X03484 CAA27204 XM_005265355 XP_005265412 XM_005265357 XP_005265414 XM_005265358 XP_005265415 XM_005265359 XP_005265416 XM_005265360 XP_005265417 XM_011533974 XP_011532276 XM_011533975 XP_011532277 NM_002880 NP_002871
Function	Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2- antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in mot
Subcellular Location	Cytoplasm. Cell membrane. Mitochondrion. Nucleus. Note=Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cell membrane by the active Ras protein. Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its mitochondrial localization. Retinoic acid- induced Ser-621 phosphorylated form of RAF1 is predominantly localized at the nucleus.
Tissue Specificity	In skeletal muscle, isoform 1 is more abundant than isoform 2. {ECO:0000269|PubMed:1886707}.
Top Pathways	VEGF signaling pathway, Focal adhesion, Gap junction, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway

• Antibodies
• Assays/Kits
• Proteins/Peptides
• siRNAs/Functional Genomics
• cDNA Clones
• Cell/Tissue/Blood Products

SignalSilence ® c-Raf siRNA II - 12342 from Cell Signaling Technology Category siRNA Prices $249.00 Sizes 300 µl (3 nmol)

SignalSilence ® c-Raf siRNA I - 12256 from Cell Signaling Technology Category siRNA Prices $256.00 Sizes 300 µl (3 nmol) Species Reactivities Human

SignalSilence® c-Raf siRNA (Human Specific) - 6281 from Cell Signaling Technology Category siRNA Species Reactivities Human

Raf-1 CRISPR/Cas9 KO Plasmid (h) - sc-400202 from Santa Cruz Biotechnology Category CRISPR Prices $345.00 Sizes 20 µg Species Reactivities Human

Raf-1 HDR Plasmid (h) - sc-400202-HDR from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

Raf-1 Double Nickase Plasmid (h) - sc-400202-NIC from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

Raf-1 Double Nickase Plasmid (h2) - sc-400202-NIC-2 from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

Raf-1 CRISPR Activation Plasmid (h) - sc-400202-ACT from Santa Cruz Biotechnology Category CRISPR Prices $345.00 Sizes 20 µg Species Reactivities Human

Raf-1 CRISPR Activation Plasmid (h2) - sc-400202-ACT-2 from Santa Cruz Biotechnology Category CRISPR Prices $345.00 Sizes 20 µg Species Reactivities Human

Raf-1 Lentiviral Activation Particles (h) - sc-400202-LAC from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

Raf-1 Lentiviral Activation Particles (h2) - sc-400202-LAC-2 from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

Raf-1 siRNA (h) - sc-29462 from Santa Cruz Biotechnology Category siRNA Prices $258.00 Sizes 10 µm Species Reactivities Human

Raf-1 shRNA Plasmid (h) - sc-29462-SH from Santa Cruz Biotechnology Category siRNA Prices $258.00 Sizes 10 µm Species Reactivities Human

Raf-1 shRNA (h) Lentiviral Particles - sc-29462-V from Santa Cruz Biotechnology Category siRNA Prices $258.00 Sizes 10 µm Species Reactivities Human

Raf-1 RNAi - H00005894-R01V from Novus Biologicals Category siRNA Prices $399.00 Sizes 20 nmol Species Reactivities Human

Raf-1 RNAi - H00005894-R03 from Novus Biologicals Category siRNA Prices $399.00 Sizes 20 nmol Species Reactivities Human

MISSION® esiRNA - EHU050131 from Sigma-Aldrich Category siRNA Prices $140.00, $250.00 Sizes 20 µg, 50 µg

MISSION® siRNA - SIHK1923 from Sigma-Aldrich Category siRNA Prices $25.00 Sizes 0.25 nmol

MISSION® siRNA - SIHK1921 from Sigma-Aldrich Category siRNA Prices $25.00 Sizes 0.25 nmol

MISSION® siRNA - SIHK1922 from Sigma-Aldrich Category siRNA Prices $25.00 Sizes 0.25 nmol

CompoZr® Knockout ZFN Kit - CKOZFN1353 from Sigma-Aldrich Category ZFN Kit Prices $4,999.00 Sizes 1 kt

CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND1353 from Sigma-Aldrich Category ZFN Kit Prices $3,999.00 Sizes 1 kt

MISSION® 3′UTR Lenti GoClone™ - HUTR07378 from Sigma-Aldrich Category miRNA Prices $350.00 Sizes hutr07378

RAF1 siRNA (Human) - CRH3983 from Cohesion Biosciences Category siRNA Prices $340.00, $510.00 Sizes 15 nmol, 30 nmol Species Reactivities Human

RAF1 - MBS8232776 from MyBioSource Prices $365.00, $515.00 Sizes 15 nmol, 30 nmol Species Reactivities Human

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