RPRD1B

Gene Symbol RPRD1B
Entrez Gene 58490
Alt Symbol C20orf77, CREPT, NET60, dJ1057B20.2
Species Human
Gene Type protein-coding
Description regulation of nuclear pre-mRNA domain containing 1B
Other Description cell cycle-related and expression-elevated protein in tumor|cell-cycle related and expression-elevated protein in tumor|regulation of nuclear pre-mRNA domain-containing protein 1B
Swissprots Q1WDE7 Q9NQG5 Q6PKF4
Accessions EAW76045 EAW76046 Q9NQG5 AK092567 AK126484 AK312468 BAG35375 AL080224 AL117521 CAH10720 BC001696 AAH01696 BC020939 BC033629 AAH33629 BP359144 DQ372938 ABD34791 XM_005260477 XP_005260534 XM_005260480 XP_005260537 XM_011528954 XP_011527256 XR_936597 NM_021215 NP_067038
Function Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. Transcriptional regulator which enhances expression of CCND1. Promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. Prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation. {ECO:0000269|PubMed:22231121, ECO:0000269|PubMed:22264791}.
Subcellular Location Nucleus {ECO:0000269|PubMed:22264791}.
Tissue Specificity Preferentially expressed in a range of tumor tissues including colon, lung, liver, breast, prostate, stomach, uterine endometrium and cervical cancers with higher levels in tumors than in adjacent non-tumor tissue (at protein level). {ECO:0000269|PubMed:22264791}.

Anti-C20orf77 antibody - ab154910 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-C20orf77 antibody (ab154910) at 1/1000 dilutionLane 1 : 293T whole cell lysateLane 2 : HeLa whole cell lysateLane 3 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-C20orf77 antibody - ab179783 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-C20orf77 antibody (ab179783) at 1 µg/ml + Mouse testis lysate at 10 µg

Anti-C20orf77 antibody - ab137246 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-C20orf77 antibody (ab137246) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : 293T whole cell lysate at 50 µgLane 4 : Jurkat whole cell lysate at 50 µgLane 5 : mouse NIH3T3 cells at 50 µgdeveloped using the ECL technique

Anti-C20orf77 antibody - ab104222 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RPRD1B Antibody (aa254-282, AP) - LS-C223935 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RPRD1B Antibody (aa254-282, APC) - LS-C223937 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RPRD1B Antibody (aa143-192) - LS-C112068 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
RPRD1B antibody LS-C112068 Western blot of Jurkat lysate.

Anti-RPRD1B Antibody (aa112-161) - LS-C201702 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of Mouse Testis. Rprd1b antibody dilution 1.0 ug/ml.

Anti-RPRD1B Antibody (aa254-282) - LS-C162726 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of RPR1B Antibody in Jurkat cell line lysates (35 ug/lane). RPR1B (arrow) was detected using the purified antibody.

Anti-RPRD1B Antibody (aa254-282, Biotin) - LS-C223938 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RPRD1B Antibody (aa254-282, FITC) - LS-C223939 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RPRD1B Antibody (aa254-282, Azide-free, HRP) - LS-C223940 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RPRD1B Antibody (aa254-282, PE) - LS-C223941 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RPRD1B Antibody (aa261-290) - LS-C324808 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RPRD1B Antibody (aa276-326) - LS-C290436 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse RPRD1B by Western Blot (h and m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RPRD1B antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. RPRD1B was also immunoprecipitated by rabbit anti-RPRD1B antibody BL13606, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Anti-RPRD1B Antibody (clone 1C9) - LS-C338483 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1C9
IHC of paraffin-embedded Human pancreas tissue using anti-RPRD1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

RPRD1B Antibody - NBP2-20222 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: RPRD1B Antibody [NBP2-20222] - Sample (30 ug of whole cell lysate) A: 293T B: HeLa C: HepG2 10% SDS PAGE gel, diluted at 1:1000.

RPRD1B Antibody - NBP1-55467 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal

RPRD1B Antibody (4D6) - H00058490-M04 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4D6
Western Blot: RPRD1B Antibody (4D6) [H00058490-M04] - Analysis of RPRD1B expression in IMR-32.

CREPT Polyclonal Antibody - bs-9971R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RPRD1B antibody produced in rabbit - SAB2701444 from Sigma-Aldrich

Prices $326.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-RPRD1B: <B>Cat. No. SAB2701444</B>: Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using RPRD1B antibody at 1:500 dilution.

Anti-RPRD1B (157-170) antibody produced in rabbit - SAB1102247 from Sigma-Aldrich

Prices $298.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human RPRD1B was separated on SDS-PAGE, blotted with Anti-RPRD1B (157-170) (<B>Cat. No. SAB1102247</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000 <BR/>2. Antibody dilution 1:1,000 + RPRD1B immunizing peptide (human, 157-170)

ANTI-RPR1B(C-TERMINAL) antibody produced in rabbit - SAB1306149 from Sigma-Aldrich

Prices $326.00
Sizes 40 tst
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-RPR1B (C-term): <B>Cat. No. SAB1306149</B>: Western blot analysis of RPR1B Antibody (C-term) in Jurkat cell line lysates (35 μg/lane).

Monoclonal Anti-C20ORF77 antibody produced in mouse - SAB1402614 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4D6
<B>Immunoblotting</B><BR/>Monoclonal Anti-C20orf77, antibody produced in mouse, clone 4D6, purified immunoglobulin: <B>Cat. No. SAB1402614</B>: Antibody concentration: 1 μg/mL. Specific band of ~61.97 kDa using immunogen protein lysate.

RPRD1B antibody - middle region (ARP57534_P050) - ARP57534_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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