| Gene Symbol |
RNF8
|
| Entrez Gene |
9025
|
| Alt Symbol |
hRNF8
|
| Species |
Human
|
| Gene Type |
protein-coding
|
| Description |
ring finger protein 8, E3 ubiquitin protein ligase
|
| Other Description |
C3HC4-type zinc finger protein|E3 ubiquitin-protein ligase RNF8|UBC13/UEV-interacting ring finger protein|ring finger protein (C3HC4 type) 8
|
| Swissprots |
A8MYC0 A6NN24 O76064 Q5NKW5 Q53H16 B4DPG0
|
| Accessions |
BAA33557 EAX03943 EAX03944 EAX03945 O76064 AB014546 BAA31621 AF334675 AAQ14887 AK022075 AK222765 BAD96485 AK298319 BAG60572 AK308840 AL711279 BC007517 AAH07517 BE327572 BF110581 BG029939 BM979512 BT007446 AAP36114 CA418096 DA064222 DQ890737 ABM81663 DQ893909 ABM84835 XM_006715241 XP_006715304 XM_006715242 XP_006715305 XR_427852 XR_427853 XR_427854 XR_427855 XR_427856 XR_427857 XR_926336 NM_003958 NP_003949 NM_183078 NP_898901 NR_046399
|
| Function |
E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'- linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites. Following DNA DSBs, it is recruited to the sites of damage by ATM-phosphorylated MDC1 and catalyzes the 'Lys-63'-linked ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). Also controls the recruitment of UIMC1-BRCC3 (RAP80-BRCC36) and PAXIP1/PTIP to DNA damage sites. Also recruited at DNA interstrand cross-links (ICLs) sites and catalyzes 'Lys-63'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20/C1orf86 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-de
|
| Subcellular Location |
Nucleus. Midbody. Chromosome, telomere {ECO:0000250}. Note=Recruited at uncapped telomeres (By similarity). Following DNA double-strand breaks, recruited to the sites of damage. During prophase, concomitant with nuclear envelope breakdown, localizes throughout the cell, with a dotted pattern. In telophase, again in the nucleus and also with a discrete dotted pattern in the cytoplasm. In late telophase and during cytokinesis, localizes in the midbody of the tubulin bridge joining the daughter cells. Does not seem to be associated with condensed chromosomes at any time during the cell cycle. {ECO:0000250}.
|
| Tissue Specificity |
Ubiquitous. In fetal tissues, highest expression in brain, thymus and liver. In adult tissues, highest levels in brain and testis, lowest levels in peripheral blood cells. {ECO:0000269|PubMed:16215985, ECO:0000269|PubMed:9852682}.
|
![Western Blot: RNF8 Overexpression Lysate (Adult Normal) [NBL1-15460] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RNF8.](http://www.bioprodhub.com/system/product_images/cel_products/5/sub_2/9239_RNF8%2520Overexpression%2520Lysate%2520(Adult%2520Normal)-Western%2520Blot-NBL1-15460-img0002.jpg)