Figure 1: Treatment of A-204 cells with Human Basic Fibroblast Growth Factor (hFGF basic/FGF2) #8910 stimulates tyrosine phosphorylation of FGFR1, as detected by the PathScan ® Phospho-FGF Receptor 1 (panTyr) Sandwich ELISA Kit, but does not affect the level of total FGFR1 detected by the PathScan ® Total FGF Receptor 1 Sandwich ELISA Kit #12828. Absorbance at 450 nm is shown in the top figure while corresponding western blots using FGF Receptor 1 (D8E4) XP ® Rabbit mAb #9740 (left) and Phospho-FGF Receptor (Tyr653/654) Antibody #3471 (right) are shown in the bottom figure.
Figure 2: The relationship between protein concentration of lysates from untreated and hFGF basic-treated A-204 cells and the absorbance at 450 nm as detected by PathScan ® Phospho-FGF Receptor 1 (panTyr) Sandwich ELISA Kit is shown. Unstarved A-204 cells (85% confluence) were treated with Human Basic Fibroblast Growth Factor (hFGF basic/FGF2) #8910 (100 ng/ml) for 2-5 min at 37°C and then lysed.