Detection of total AKT1 in MCF7 cells treated with various concentration of wortmannin, using ab119616.
Using the AKT1 ELISA kits, a significant increase in AKT phosphorylation at Ser473 is detected in MCF7 cells treated with insulin for 15 minutes (+), compared with untreated cells (-), whereas no change in total AKT 1 levels was observed.
Using Western blot, a significant increase in AKT phosphorylation at Ser473 is detected in MCF7 cells treated with insulin for 15 minutes (+), compared with untreated cells (-), whereas no change in total AKT 1 levels was observed.