Using the CREB1 assay kits CREB1 phosphorylation at Ser133 is detected in EGF-treated A431 cells (+), compared with untreated cells (-), whereas no change in total CREB1 levels was observed.
Using the CREB1 western blot, CREB1 phosphorylation at Ser133 is detected in EGF-treated A431 cells (+), compared with untreated cells (-), whereas no change in total CREB1 levels was observed.
A significant increase in AKT Ser473 phosphorylation is detected in MCF7 cells treated with insulin for 15 minutes (+), compared with cells treated with wortmannin and LY294002 (-), whereas no change in total AKT 1 levels was observed.
A significant increase in AKT Ser473 phosphorylation is detected in MCF7 cells treated with insulin for 15 minutes (+), compared with cells treated with wortmannin and LY294002 (-), whereas no change in total AKT 1 levels was observed.
Using the ERK 1/2 assay kits, a significant reduction of ERK 1/2 phosphorylation is detected in A431 cells treated with AG1478 for 3 hours compared with untreated cells, while only a small change in total ERK levels is observed.
Using the ERK 1/2 western blot, a significant reduction of ERK 1/2 phosphorylation is detected in A431 cells treated with AG1478 for 3 hours compared with untreated cells, while only a small change in total ERK levels is observed.