| Description |
Cellular inhibitor of apoptosis protein 1 (cIAP-1, also known as BIRC2, MIHB, and HIAP2) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. Structurally, cIAP-1 is comprised of 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). Functionally, cIAP-1 inhibits caspases through the direct interaction of its BIR domain with the active caspase. The ring finger domain of cIAP-1 also functions as an E3 ubiquitin ligase to ubiquitinate specific target proteins. Caspase activity may be restored by mitochondrial proteins, such as SMAC/Diablo or HtrA2/Omi, through interactions with the Reaper-like motif and the BIR domain. This kit is designed for in vitro cIAP-mediated ubiquitination of user-supplied substrates. Ubiquitinated proteins can be used in downstream applications, or analyzed by Western blot using antibodies specific for the target protein. A detection antibody ( AF8181 ) is included in the kit to monitor autoubiquitination of cIAP-1. NOTE: Kit contains reagents sufficient for 10 x 30 μl reactions and 5 Western Blots (mini-gel format).
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