PathScan ® Phospho-Chk1 (Ser317) Sandwich ELISA Kit
| Name | PathScan ® Phospho-Chk1 (Ser317) Sandwich ELISA Kit |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 7870 |
| Category | ELISA Kit |
| Prices | $489.00 |
| Sizes | 1 kit (96 assays) |
| Assay Type | Sandwich |
| Applications | ELISA |
| Species Reactivities | Human |
| Description | The PathScan ® Phospho-Chk1 (Ser317) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Chk1 when phosphorylated at Ser317 |
| Gene | CHEK1 |
| Supplier Page | Shop |
Product images
Figure 1. Treatment of HeLa cells with UV stimulates phosphorylation of Chk1 at Ser317, detected by the PathScan® Phospho-Chk1 (Ser317) Sandwich ELISA Kit #7870, but does not affect the levels of total Chk1 detected by PathScan® Total Chk1 Sandwich ELISA Kit #7872. HeLa cells (80-90% confluent) were treated with 100 mJ/cm2 UV with 1 hour recovery at 37ºC. The absorbance readings at 450 nm are shown in the top figure, while the corresponding Western blots using Chk1 Antibody #2345 (left panel) or Phospho-Chk1 (Ser317) Antibody #2344 (right panel) are shown in the bottom figure.
Figure 2. The relationship between the protein concentration of lysates from untreated and UV-treated HeLa cells and the absorbance at 450 nm using the PathScan® Phospho-Chk1 (Ser317) Sandwich ELISA Kit #7870 is shown.